Shenqi Pill Treatment Of Spleen&Kidney Yang-deficiency Type Of Experimental Study Of Peptic Ulcer

Peptic ulcer(peptic ulcer) refers to the gastrointestinal tract by gastric mucosa and the formation of digestive juice digestive submucosa as deep as the mucosa is damaged,can occur in the esophagus,stomach,duodenum and gastrojejunostomy mouth of the diverticulum with gastric mucosa inside. Clinical to gastric ulcer(gastric ulcer,GU) and duodenal ulcer(duodenal ulcer,DU) common,it generally refers to the so-called peptic ulcer GU and DU.Peptic ulcer in Chinese medicine as "Epigastralgia" areas for the disease pathogenesis,although views vary,but most doctors believe was mainly due to the impassioned stimulation,diet,internal injuries,as well as exogenous tired Six,lead to weak spleen and stomach,hepatogastric not and,thus lifting disorders,gastrointestinal injury,a long time from disease."Neijing" pointed out:"Since the times of eating,gastrointestinal injury is," "think of spleen injury," "cold off at the stomach,the Jue-Inverse out,it is also pain and vomit." "Jing Yue Quan Shu" that "the spleen and stomach injuries in the emotions of those who,for more years than eating it." Cold spleen and stomach,then violations by the liver,Violations by the liver-qi to the stomach,spleen and stomach are more weak and more a long time while yang is true,the evidence becomes cold.And weakness in the blood gas barrier, Blood Meridian,the pain is not General.Judging from the clinical observation,regardless of what type of peptic ulcer,are mainly pain,gastrointestinal pathology from the point of view, is the main mechanism of gastrointestinal motility dysfunction.From the TCM perspective,is the deficiency,a series of symptoms of Qi stagnation.Its pathogenesis pathological movements mainly the spleen and stomach disorders, deficiency,Qi stagnation,blood stasis.Physiological,spleen governs transport and satisfied by the main stomach and spleen health to be promoted. Down to the stomach and,landing and taking off in an orderly manner,then reconcile Qi,Wei Qi is the spleen and stomach Tongjiang an important part of movements in an orderly manner.In treatment,whether in the goal of TCM treatment of peptic ulcer is three-fold:(1) pain;(2) to promote ulcer healing as soon as possible;(3) to prevent recurrence.In Western medicine is re-active ulcer ulcer repair.Against the patient's individual physique,to the TCM treatment is particularly important.Differential Treatment of clinical focused to the main deficiency,treatment is mainly Jianpi Yiqi,and promoting blood circulation,mainly to stagnation of qi,the hardship of the liver and spleen.Grams of phase insult each other constraints,the spleen and stomach qi deficiency,liver by soil.Ye Tianshi's said:" Violations of the liver to the spleen and stomach are sick stomach." Wei Qi stagnation of liver Qi stagnation or Pause.From the qi and blood differentiation aspects of treatment,can relieve pain,promote healing and prevent ulcer recurrence and derived from the tripartite and.In favor of the spleen and stomach function returned to normal.Objective:Shenqi Pill use in the past,based on the use of modern scientific theories, and experimental study of scientific and technological means to expand its scope of application.Through experimental research,from the histological observation,pathological observation,gastric analysis,thequality of ulcer healing(QOUH) Shenqi PillⅣstudy the mechanism of the treatment of peptic ulcer and target,revealed Shenqi Pill mucosal injury and the treatment of peptic ulcer the specific mechanism of regeneration,in order to provide scientific basis for clinical application.Shenqi Pill evaluation of multi-angle on a variety of common peptic ulcer effects model to examine the role of targets and the rationale for the treatment of peptic ulcer Busben law to provide a reasonable scientific basis to enrich the government, "stomach" Disease Treatment and expand ideas of TCM diagnosis Methods:The model approach1.The establishment of animal models of kidney-yang deficiency of spleen2.Spleen and kidney yang deficiency rats with gastric ulcer3.Animal grouping and drug delivery:were randomly divided into 5 groups of 10:A model group;B.saline control group;C.ranitidine group;D.Shenqi pill group;E.Shenqi Pill +RIRr group;. 4.Specimen collection and processing(1) the animals from a group of kidney-yang deficiency of spleen acid burn caused by gastric ulcer,the bacteria can not stomach the determination of other indicators measured.Division and with the former model.All animal experiments to be together after the test by radioimmunoassay PGE2,EGF,SS and GAS,NO and ET-1 expression.(2) gastric acid and pepsin determination of:(A) Determination of gastric juice and total acidity(B) Determination of improved Mett pepsin activity(3) combined gastric wall mucus determination5.Observed gastric pH;ulcer area;serum PGE2,EGF,GAS,NO and ET-1 content;pathological observation,and analysis of optical microscope.6.Statistical data using SPSS 13.0 statistical measurement data to compare the use of multiple analysis of variance,22 inter-group homogeneity when compared with LSD test at variance with missing Dunnett's T3 test,the use of count data X2 test.The results:1.To observe the group of gastric wall mucus content of combined results showed that:the use of computer image analysis measuring the total area of mucosal regeneration in the percentage of PAS-positive area.The results of gastric mucosa surface epithelial cells and mucus secretion by neutral red dye,it was PAS positive,ranitidine group,Shenqi Pill group Shenqi pill + RIRr Group PAS-positive area significantly more than the model group(P＜0.05), the results shown in Table 3,Figure 1.2.In each group of serum EGF,GAS showed the impact:①the model group with Shenqi pill + RIRr value between the level of EGF significant difference, (P＜0.01);②GAS few in each group were in line with the side poor homogeneity, but there is no difference between groups statistically significant.The results in table 4.Each group on serum PGE2,NO,Er-1 results showed the impact:①the model group and the ranitidine group,Shenqi Pill group Shenqi pill + RIRr group difference between the PGE2 levels significantly,(P＜0.05).Ranitidine group after treatment,Shenqi Pill group Shenqi pill + RIRr group decreased significantly;②model group and the ranitidine group,Shenqi Pill group Shenqi pill + RIRr value between the level of NO There was significant difference,(P＜0.05).Ranitidine group after treatment,Shenqi Pill group Shenqi pill + RIRr group was significantly increased.③the medication group, model group,normal saline control group between the serum ET-1 compared with model group,treatment group comparisons are statistically significant, ranitidine group after treatment,Shenqi Pill Group Shenqi pill + RIRr group decreased significantly,which Shenqi pill + RIRr group was significant(P＜0.01).The results in table 5.Conclusion:The Shenqi Pill pharmacological research reports from the system or of a lesion index of a start,observe the side effects of treatment and to explore the mechanism of action.In the experiment,more than modern medicine in accordance with animal models of pathogenesis for a copy of the original animal model or in some improvements based on the production of pathological model, the content from the observation of symptoms,vital signs,morphology and histology,the immune and gradually into the biochemical indicators of gene expression.While this approach can reveal a certain level,some of the role of Shenqi Pill target and impact of the mechanism,but because of characteristics of syndrome differentiation in Chinese medicine,under the guidance of the overall regulation of the "disease" as the focus of experimental study on pharmacology of Chinese medicine obviously with the Chinese "differentiation agents" a far cry from the treatment guidelines. Although the majority of scholars in the TCM Syndrome animal model of an active area of research for the exploration,but the maturity of the animal model findings rare.From the future to improve the animal model can start to explore the disease and modern medicine of TCM Syndrome Types of the intrinsic link between the establishment of the objective of TCM Syndrome comprehensive index system,and then replicate with the index system of TCM Syndrome animal model, from the side perspective permits the corresponding drug,based on a whole will be multi-layered,multi-target detection of indicators included in the evaluation criteria of drug efficacy,the use of gene chips and other modern science and technology,from multi-disciplinary,multi-system perspective of the health of drug treatment mechanism for an accurate grasp of the role of Chinese herbal compound in vivo,as well as objective evaluation of the principle of Chinese medicine composed of government inspection results,will be more said,and help to promote the development of Chinese medicine modernization.Innovation1.'s First at home and abroad of a combination of Chinese medicine pill side Shenqi precious Tibetan medicine in RIRr to experimental research. Evaluation and proof of participation Shenqi Pill and hand treatment of peptic ulcer spleen rat model of yang-deficiency type of effect and found that the possible role of its mechanism and target in the treatment of peptic Shenqi Pill reveal the mechanism for further gene and protein expression studies to lay the foundation for the clinical application provides a scientific basis.2.For peptic ulcer treatment at home from the spleen and stomach,from the Liver,fresh from the kidney of the ruler.We used rat animal model of TCM Syndrome model for ways to build on the spleen yang-deficiency type of peptic ulcer model,from the histological observation,pathological observation,gastric analysis and cytokine detection,the evaluation of treatment of spleen and kidney Shenqi Pill Yang-type mechanism of peptic ulcer, is the first method for the treatment of peptic ulcer Bushen experimental study of animals.