Study On The Chemical Constituents And Anti-hpaiv Activity Of Galium Aparine

Infections with influenza A viruses still pose a major threat to humans and several animal species. The occurrence of highly pathogenic avian influenza viruses (HPAIV) of the H5N1 subtype capable to infect and kill humans highlights the urgent need for new and efficient countermeasures against this viral disease. In the dissertation, we demonstrate that the extract from the Galium aparine exerts a potent anti-influenza virus activity in MDCK cell cultures infected with H5N1 subtype.Galium aparine is a plant of the Rubiaceae family, distributed in the southern provinces of China. It is used in traditional Chinese folk medicine for the treatment of influenza. However, the investigations on the chemical constituents of plant have been few.In a search for the active constituents from the plant, the 60% ethanol extract of the plant was subjected to the systematical chemical investigation, leading to the isolation of thirty-three compounds by chromatography methods. And twenty-six structures were elucidated on the basis of physical and chemical evidences, and modern spectral analysis (including IR,UV,1H-NMR,13C-NMR,HMQC,HMBC,DEPT,EI-MS,et al.). Their structures were elucidated as deacetyl asperulosidic acid (1),2-carbometho-xy-3-prenyl-1,4-naphthohydroquinone-1-O-β-D-xylopyranoside (2), 2-carbomethoxy-3-prenyl-1,4-naphthohydroquinone-di-β-D-glucoside(3), 2-methyl- 1,3,6-trihydroxy-9,10-anthraquinone-O-α-rhamnosyl(1→2)-β-D-glucoside(4), xantho- purpurin (5), cleamastanin B (6), quercetin (7),luteolin (8) ,apigenin (9), linarin (10), kaempferol-3-O-3-L-rhamnopyranosyl(1→2)-O-β-D-glucopyranoside (11),tamarixeti- n-3-rutinoside (12), uracil (13), cytosine (14), uridin (15), cytidine (16), thymidine (17), adenine-riboside (18), p-hydroxybenzcic acid (19), protocatechuic acid (20), vanillic acid (21), caffeic acid (22) ,β-sitosterol (23),β-daucosterol (24), n-hexadecane (25). azelaic acid (26). Compound 2 was a new compound. The result of the research was meaningful to a widespread usage of the plant, it is also helpful for the development of a new medicine.At the effective dose of 37.5μg/mL, the compound 1 did not exhibit apparent harming effects on cell viability, metabolism or proliferation, which exerted a potent anti-influenza virus activity in MDCK cell cultures infected with H5N1 subtype.