Studies On LSPR Effect Of Assembled Gold Nanoparticles Film

Along with the rise of nanometer science and technology, nano-scale gold particles show potential applications in many fields because of their unique optical and electrical properties, which attract the interest of scientists greatly. And then, the applications of the localized surface plasmon resonance (LSPR) effect on gold nanoparticles have been a research hot-spot during recent years.In the paper, the localized surface plasmon resonance effect and the surface enhanced Raman scattering (SERS) are investigated, and a new biosensor to monitor the real-time biomolecular interaction dynamically is designed based on LSPR effect of gold nanoparticles film. The new biosensor can monitor the biomolecular interaction in real time and in situation, without using any labels. It can be used to study interactions between many species, and to continuously monitor the process of adsorption and desorption as wel1 as association and dissociation; It is very sensitive to small molecules; It has high resolution of one nanoparticle; It has very little nonspecific binding; It is relatively cheap and convenient in carrying. Thus it has a wide foreground in application.In the paper, the gold nanoparticles film is fabricated based on nanosphere lithography (NSL). The gold nanoparticles are adsorbed and fixed to the surface of glass by Self-Assembled technology, and the gold nanoparticles film of submonolayer distribution is gained. The structure of gold nanoparticles film is analyzed by SEM and reflecting spectra. The SERS of gold nanoparticles film is measured, using pyridine as probe molecules, which has obvious enhancement and can reach 107.A spectroscopy acquisition system is designed depending on the LSPR. The Gauss-Lorentz fitting equation is built to gain the peak absorbtion wavelength of reflecting spectra of sample. The LSPR effect of gold nanoparticles film is analyzed by Sucrose experiment, and it shows a red-shift of peak absorbtion wavelength when the refractive index of external medium of gold nanoparticles film changes. The dynamic data acquisition and process on the reflecting spectra of gold nanoparticles film which is assembled by MUA is carried out through LabVIEW.This project is sponsored by the National Natural Science Foundation of China (Project No. 60508003) and the Tianjin Application of Basic Research Project Foundation (Project No. 06YFJMJC07100).