| Objective: To study the immunophenotype and its relationship with prognosis of acute leukemias. Method: Immunophenotypes were examined by indirect immunofluorescence method in 108 AL. Results: â‘ In 108 AL, one case of M2 ,one case of MJ and one case of LS diagnosed by FAB morphology, finally rediagnosed as acute mixed leukemia (MAL) and four cases of undifferentiated acute leukemia (UAL) were diagnosed as minimally differentiated AML (Mo)by immunophenotyping.â‘¡ALL: T-ALL 27%, B-ALL 73%, of 11.5% cases coexpressed myeloid associated antigens, among them, CD13, CD33 were the most often expressed, no difference was found in expression of myeloid antigens between T-ALL and B-ALL(P>0.1). CD34+was most frequently expressed in B-ALL than T-ALL(53%vs28%),but no significant difference was found between two groups (P>0.05) ,there were no significant difference was noted in the age, gender, peripheral blood WBC ,hepatomegaly, splenomegaly and lymphnode enlargement between T-ALL and B-ALL ,but the marrow blast cells were significantly lower in T-ALL (P0.05) .â‘¢AML: The expression rate of myeloid antigens were CD33>CD13> CD15 > CD14. 35 cases(43.2%)of AML coexpressed at least one lymphoid marker, CD7+ was the most frequently expressed(22.8%), 13.9% of cases CD4+, 8.9% of cases CD,94and 5.1% of cases CD2+.The early stem cell markers such as CD34, CD38, HLA-DR were highly expressed in CD7+- AML , CD7+was rarely expressed in Ma but highly expressed in M0 M1 (P0.01) comparing CD7+-AML with CD7--AML, the former had lower CR rates (50%vs62.5%) .but no significant difference between them was noted. CD34+ was detected on 42% of AML and the expression rate of CD34+was significantly higher in M0 M1 than the other subtypes ( P<0.01 ) .The CR rate significantly lower in CD38+-AMLthan CD38--AML (P<0.05) . Conclusion: There was a significant value of immunophenotyping at the diagnosis of MAL and Mo-The expression of CD7 might predict a poor clinical outcome in AML.
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