Immunophenotype Of Acute Leukemia And Its Relationship With Clinical Outcome

Objective: To analysis expression of lymphoid marks and CD34 antigen on myeloid blasts in AML and myeloid marks on lymphoblasts in ALL. To evaluate the relationship between the expression and chemotherapy sensitivity and CR rate.Methods: Immunopheotypes were examined using indirect immunofluoresence methods in 55 AL, including AML 34 cases, ALL 21 cases. All cases were treated with combined chemotherapy. DA, HA regime were used to treat AML; VP, VDP,VDCP regime were used to treat ALL. Then analyzed the difference of CR rate between lymphoid antigen positive AML and lymphoid antigen negative AML, the difference of CR rate between myeloid antigen positive ALL and myeloid antigen negative AL (Chi square test).Results: (1) The incidence of lymphoid antigen expression in 34 AML was 11.8%. Among them, CD7 was 8.8%, CD5was 3.0%, CD7 was mainly expression in M2, M5 subtypes. CR rate in lymphoid antigen positive AML is lower than lymphoid antigen negative AML(25.0% Vs 80% p<0.05). CR rate in CD34 positive AML is lower than in CD34 negative AML(55.6%Vs 90.5% p<0.05). CRratein CD13, CD14, CD15 positive AML is no difference from CD 13, CD 14, CD 15 negative AML(p>0.05). (2) The incidence of myeloid antigen expression in was 23.8%. Among them , CD33 was 14.3%, CD15 was 4.8%. Myeloid antigen were expressed lower in CD34 positive ALL than in CD34 negative ALL(14.3% Vs 28.6%), but there is no significant difference between them(p>0.05). CR rate in myeloid antigen positive ALL is lower than in myeloid antigen negative ALL(20.0% Vs 81.3%).Conclusion: In AML, lymphoid antigen and CD34are negative correlated with CR rate,them might predict clinical outcome; In ALL, myeloid antigen is also negative correlated with CR rate ,it can predict a poor clinical oucome.