Studies On Flavonoids From Stems And Leaves Of Panax Notoginseng

Panax Notoginseng(Burk.) F.H.chen, a well-known medicinal plant , which is widely used for treatment of cardiovascular diseases, inflammation, different body pains, trauma , bleeding caused by internal and external injury. It was been also used as a tonic and haemostatic agent. Due to the demand for its important medicinal use in China, it was largely cultivated in Yunnan Province.The saponins in this plant have been reported more before, but the flavonoids reported fewer. For the sake of the better use of this herb medicine, this paper describes the isolation, structural determinations and the analysis of the flavonids from the dried stems and leaves of Panax Notoginseng. Extraction and isolationDried stems and leaves (2kg)were extracted with water three times(30L ,24L,20L;2h,1.5h, lh).The extracted solution was absorbed by macroporous resin, then eluented with 95%EtOH. The extraction was subjected to silica gel column chromatography using methanol-chloroform gradient aseluent to afford SA (4.2g) ,SB (3.1g) ,SG (195mg) ,SH ( 22mg )and SI( 35mg ). The extraction was subjected to ODS column chromatography using methanol-water gradient as eluent to afford SC (68mg) and SD (9mg) .Compounds SA, SB, SC, SQ SH and SI was identified as kaempferol 3 - O - ( 2" - p - D - glucopyranosyl) - |3 - D- galactopyranoside, quercetin 3 - O - ( 2" - (3 - D -glucopyranosyl) - |3 - D - galactopyranoside, kaempferol 3- O - P - D - galactoside, kaempferol, quercetin, kaempferol 7-O-a-L- rhamnoside respectively by UV, IR, MS,JHNMR and 13CNMR.Compound SD is not identified now and hoped to be identified in the future .But for SH and SG ,all the compounds were isolated from Panax Notoginseng for the first time.Analysis offlavonids in the leaves of Panax Notoginseng In this paper we present a more sensitive quantificationmethod by HPLC for analyzing the main flavonoids (SA andSB) in the leaves of Panax Notoginseng. HPLC was performed on a ZORBAX C18 column (250vmmx4.6 mm,5 JJL m )at 25 癈 with the rate of 1.2 ml/mm , using 35% methanol and 65% water/acetic acid mixture (100/1 v/v) as mobile phase.The flavonol compounds SA and SB were extracted with different solvents: methanol and water .Two extraction methods have been compared in this paper. The results show that the content of SA and SB extracted with methanol was 0.20% and 0.38% respectively compared to the results 0.17% and 0.30% extracted with water.