The Expression And Comparison Of CD105 And Nestin In Lung Cancer

Objective: Angiogenesis is the propelling force for tumor growth and metastasis, and antiangiogenic therapy represents one of the most promising modalities for cancer treatment.At present,pan marker of vascular endothelial cells(EC) such as CD34,CD31 and vWF are used in the research of tumor angiogenesis.Owing to the high heterogeneity of the ECs,and the affinity of the above antibodies to the common ECs, these antibodies are lack of specificity to the neovascular ECs in tumor tissues. In this research, CD105 acted as a new marker of ECs to identify the new born vessels in the tumor tissues and to provide a new idea of research and therapy for anti-angiogenesis. The research also detected the expression of Nestin in the tissue of lung cancer, to discuss the possibility for nestin being used as a new marker of neovascularization. Method: Double immunohistochemistry was used to identify the expression of CD105,Nestin and a-SM-actin in the fresh tumor and peri-tumor tissues of 45 cases of lung cancer, Immunofluorescence was applied to identify the difference of expression between CD105 and Nestin. Result: There are significant differences in the expression of CD105 between the tumor tissues and non-tumor tissues of the same patient(P<0.001), and so with the expression of Nestin(P<0.001); Statistic significance are detected between the expression of CD105 and Nestin in the tumor tissues of the same patient, with higher expression of the former;Conclusion: 1 When malignant solid tumors grow to certain stage the intensity of microvessles in tumor tissues will seemingly exceed that of the according benige tissues. 2 CD105 is abundantly expressed in angiogenic endothelial cells(ECs) of both peritumoral and intratumoral vessels. The results of our study indicate that CD105, as a marker of angiogenic ECs, may be applied to the research of antiangiogenesis and bear theraputic potential in patients with lung cancer; 3 Although Nestin is also highly expressed in angiogenic ECs it has little relation to metastasis of lymphonode . So its clinical application needs to be further investigated. 4 It's much more seemingly to detect new born vessels in tumor tissues with double immunohistochemistic staining for a-SM-actin and markers of angiogenesis.