| Objective: Make the Protocol of serum pharmacology; Study the effect of Rubia yunnanensis on the immune function stimulated by superantigens of streptococcus.Methods: By studying the effect of serum, time of getting blood and concentration of serum, make a stable protocol of serum pharmacology. Using cell culture technique, mouse spleeny lymphocytes and peritoneal macrophages were culture with superantigens of streptococcus and serum of Rubia yunnanensis. Lymphocyte proliferation stimulated by superantigens of streptococcus measured by MTT assay. NO level in mouse peritoneal macrophages affected by Rubia yunnanensis at different concentrations were determined and compared. Moreover, we also study the effects of cellular immunity of Rubia yunnanensis.Results: serum itself can also inhibit proliferation of T cell; Serum taken 1h or 1.5h later has higher concentration of effective elements. Serum of Rubia yunnanensis significantly inhibits the proliferation of lymphocytes stimulated by superantigens of streptococcus, compared with normal control. NO content of macrophages culture in Rubia yunnanensis extract group were lower than those of control. In these tests, the effect of the extract by alcohol is more obvious than that of extract of water.Conclusion: The best protocol of serum pharmacology: bid ig; get blood after lh; add 10% serum into culture. Rubia yunnanensis extract has effect in inhibiting proliferation of T lymphocytes and secretion of NO from macrophages stimulated with streptococcal antigens. It could be applied in clinical practice for immunoregulation and treatment of psoriasis.
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