Pharmacological Study On Compound Schisandra Oral Liquid

Backgroud and purpose:Compound schisandra oral liquid(CSOL)is composed of schisandra (principal drug), jujube seed (jujube seed) and other Chinese crude drugs according to the theory of traditional Chinese medicine. In recent years, the research of schisandra and its compound prescription have been achieved great progress, attracted much attention, and found extensive clinical applications and health-promoting. This article is designed to investigate sedative/hypnotic, free radical scavenging and hepato-protective effects of CSOL via animal experiments in vitro and in vivo, in order to provide further preclinical pharmacological basis for its development and clinical uses.Methods:1.In vitro free radical experiment: Hydroxyl free radical (?OH) induced mouse hepatocellular lipid peroxidation model and ?OH induced rabbit RBC membrane rupture model were used to study CSOL in vitro anti-free radical effect and protective effect against oxidation-stress caused cell injury. Determination index include the scavenging rate of ?OH, the inhibitory rate of hepatocellular lipid peroxidation and the RBC membrane rupture.2.Central Nervous System experiment: The sedative and hypnotic effects of CSOL on CNS were studied by use of mouse spontaneous activity and pentobarbital body-righting reflex models. The determination index includes time of movement and number of raising up of mouse upper extremities in limited time, and the latent period and duration of mouse sleep.3.In vivo mouse liver damage experiment: The liver damage model is induced by N-acetyl-P- aminophenol (AAP). At 24h after ig AAP(500mg/kg), theblood and liver were removed immediately. GPT activity and LDH activity in serum, as well as liver MDA content were measured.Results:1.In vitro anti-free radical effect: When the reaction system was treated with high(0.375g/ml),middle(0.250g/ml ) and low(0.167g/ml )concentration of CSOL, the scavenging rate of ?OH generated by Fenton reaction was found to be 32.73%, 20.12% and 0.90%, respectively. The inhibitory rate of ?OH induced hepatocellular lipid peroxidation was 66.51%, 51.13% and 13.41%, respectively, at concentration of 0.047g/ml, 0.023g/ml and 0.012g/ml. The pretreatment with CSOL at concentration of 0.375g/ml, 0.188g/ml and 0.094g/ml resulted in attenn rupture of RBC membrane with the inhibitory rate of 96.35%, 31.38% and 11.24%, respectively.2.Central Nervous System effect: CSOL had sedative and hypnotic effects in mice receiving ip and po. CSOL, as evidenced by shortened time of movement and decreased number of raising up of mouse upper extremety as well as the potentiated effet of Pentobarbital induced sleep. The above findings were demonstrated in a dose-dependent manner. 3.In vivo protective effect against liver damage: the activity of GPT and LDH in serum and MDA content of liver tissue were significantly dropped in CSOL treated group as compired with that in the sham group.Conclusion:1.CSOL shows extremely potent free radical-scavenging effects.2.CSOL has obvious sedative and hypnotic effect.3.CSOL has obvious protective effect on AAP-induced mouse liver damage.