Anti-inflammatory Effects And Mechanisms Of Shaoqiduogan

TGP and ASTs have significant anti-inflammatory,anti-oxidative and immunomodulatory effects.SQDG prepared from Paeonia lactiflora and Astragalus membranaceus by modern medicine production technique demonstrates its protective action on chemical and immunological liver injury and hepatic fibrosis.It is also found that SQDG might have anti-inflammatory and immunomodulatory effects.This study uses different inflammatory models to research the anti-inflammatory effect and mechanism of SQDG. The effect is compared with TGP,TGP+ASTs to investigate the change of the pharmacodynamic action after combination of medicine.Objective:To investigate the anti-inflammatory effects and the mechanism of SQDG, and compare the effect with TGP and TGP+ASTs.Methods:The anti-inflammatory effects were observed by inflammatory models of car edema induced by dimethylbenzene in mice,hind paw edema induced by canrrageenan in rats and granuloma induced by cotton pellet in rats,then determine the content of prostaglandinE₂ in the swelling hind paw of rats to observe the mechanism.Compare the difference of the effect of SQDG with TGP,TGP+ASTs on the inflammatory models.Results:1.Effect of SQDG on ear edema induced by dimethylbenzene in mice: SQDG(30,60,120mg·kg^-1) intragastrical administrated continuously for 5 days before inflammation can inhibit the ear edema induced by dimethylbenzene in mice.The inhibitory action of SQDG(30mg·kg^-1) is better than TGP(35mg·kg^-1), TGP+ASTs(26.25+3.75mg·kg^-1).The inhibitory action of SQDG(60mg·kg^-1) is better than TGP(70mg·kg^-1),TGP+ASTs(52.5+7.5mg·kg^-1).The inhibitory action of SQDG (120mg·kg^-1) is better than TGP+ASTs(105+ 15mg·kg^-1).2.Effect of SQDG on hind paw edema induced by carrageenan in rats:SQDG(21.25,42.5,85mg·kg^-1) intragastrical administrated continuously for 5 days before inflammation can inhibit the hind paw edema induced by carrageenan in rats at the 1,3,5,7 hour after inflammation.At the 1,3,5 hour after inflammation,the inhibitory action of SQDG(21.25mg·kg^-1) is better than TGP(25mg·kg^-1),TGP+ASTs(18.5+2.75 mg·kg^-1).The inhibitory action of SQDG(42.5mg·kg^-1) is better than TGP(50mg·kg^-1) at the 1,3,5,7 hour and TGP+ASTs (37+5.5 mg·kg^-1) at the 1,3,5 hour after inflammation.The inhibitory action of SQDG(85mg·kg^-1) is better than TGP(100mg·kg^-1) at the 1,3,5,7 hour after inflammation.SQDG(21.25,42.5,85mg·kg^-1) could reduce the content of prostaglandinE₂ in the swelling hind paw of rat. The inhibitory action of SQDG(21.25mg·kg^-1) is better than TGP(25mg·kg^-1), TGP+ASTs(18.5+2.75mg·kg^-1).The inhibitory action of SQDG(42.5mg·kg^-1) is better than TGP(50mg·kg^-1),TGP+ASTs(37+5.5mg·kg^-1).The inhibitory action of SQDG(85 mg·kg^-1) is better than TGP(100mg·kg^-1).3.Effect of SQDG on granuloma induced by cotton pellet in rats:SQDG(21.25,42.5,85mg·kg^-1) intragastrical administrated continuously for 7 days after inflammation can inhibit the formation of granuloma induced by dimethylbenzene in rats.There is no significant difference between SQDG(21.25mg·kg^-1) and TGP(25mg·kg^-1), TGP+ASTs(18.5+2.75mg·kg^-1).The inhibitory action of SQDG(42.5mg·kg^-1) is better than TGP(50mg·kg^-1).The inhibitory action of SQDG(85mg·kg^-1) is better than TGP (100mg·kg^-1),TGP+ASTs(74+11 mg·kg^-1).Conclusion:SQDG has significant anti-inflammatory effects.The inhibitory actions on ear edema induced by dimethylbenzene in mice,hind paw edema induced by carrageenan in rats and granuloma formation induced by cotton pellet in rats are better than TGP and TGP+ASTs.The mechanism of anti-inflammation is related to reducing the lever of PGE₂.