Experimental Studies On The Shengjingsan Recipe's Effects For The Treatment Of A Rat Model Of Asthenozoospermia

Asthenospermia common cause of male infertility is caused one.The etiology is unknown, the pathogenesis is not clear,in the absence of effective clinical treatment.The basis of our previous work showed that the ancient Chines Asthwnospermia common cause of male infertility is caused by e medicine side,"Wu Zi Yan Zong Wan",modified form the basis of the"germ power"formular ,for the treatment of low sperm has a good effect.but the mechanism is unclear. The study is to graduate from basic aspects to this fine power on the efficacy of weak sperm and therapeutia mechanism ,first pro thai the sperm-specific calcium channel protein Catsper expression or dysfunction is an important cause disease asthenospermia one of the mechanisms set out the weak pathogenesis of sperm ,on this basis ,demonstrate that Shengjingsn can enhancet sperm motility ,fertilization ability of hyperactivation by activatied sperm-specific Catsper channel .and to clarify the Shengjiansan on the efficacy of Asthenospermia and its treatment mechanism.The present study ,the method of feeding GTW induced the occurrence of weak sperm ,the use of testicular histopathological(HE staining),senmen analysis and sperm motility conventional detection methods ,detection of rat sperm morphology and vitality ,the establishment of weak sperm disease rat model ,while using Real-time PCR(mRNA level of detection )and immunohistochemical method to observe the four Catsper channel protein(Catsper1-4) in the model of the espression of rat sperm plasma membrane changes,trying to clarify the following questions :1)four Catsper channel protein (Catsper1-4) in the rat model whether the expression of sperm plasma memebrane decreased compared with the control goup;2) whether the Shengjinsan can enhance the function of Catsper channel of model rat by increasing the expression of the sperm plasma membrance to improve the vitality of the sperm.1.Building the rat model asrhenospermia (GTW)Using the method of feeding GTW induced by the oocurrence of asthenospermia ,the use of testicular histopathological (HE staining).semen analysis and sperm motility conventional detection methods,detetion of rat sperm morphology and vitality .The results showed that :GTW group sperm viability,vigor,density,ans linear(VSL),average path velocity (VAP) and the curve velocity(VCL) and other functional parameters were mormal control group and NS group was significantly reduced.HE staining showed that testicular tissue of model group at all level within the seminiferous tubules in spermatocytes and sperm cells was significantly reduced ,disorded arrangenment of spermatogenic cells ,indicating that GTW can induce a stable rat model of asthenospermia.2. whether have sperm-specific calcium channel Catsper protein expression of reduced in asthenospermia ratsBy immunohistochemistry ,Real-time PCR methods were observed (Catsper1-4) in the rat model whether the expression of the sperm plasma memebrane than control group decreased .The results showed that: the model group catsper protein was reduces compared with the control group.To further study the oocurrenence of weak sperm provided the basis.3. Shengjinsan with increased sperm density ,motility,viability and the role velocityBy using computer-aided sperm analysis system(CASA) detect the density of spem ,motility,viability and function of velocity ,and other indicators.results:the Shengjinsan can significantly improve the model group,the bulk of sperm density ,motility ,viability and velocity ,and thus treated rats asthenospermia .