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Adenine Method Of Yi Jing Fang Treatment Of Infertility In Rats Less The Asthenozoospermia Experimental Study

Posted on:2009-08-27Degree:MasterType:Thesis
Country:ChinaCandidate:J G ZhouFull Text:PDF
GTID:2204360272966019Subject:Chinese medicine prescription
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Objective To study the curative effects of Chinese herb yijingfang on oligospermia and asthenospermia of infertility rats leaded by adenine and analyze corresponding relationship of the dose and the curative effect.Methods Sixty SPF Wistar mature male rats were divided into five groups by their weights: the normal control group(twelve),the model control group(twelve),the treatment group by lower dose(twelve),the treatment group by middle dose(twelve),the treatment group by higher dose (twelve).Except for the normal control group, all the other four groups were perfused with adenine(50mg/100g/d)once a day for ten days, so we established male infertility models of rats. Then the treatment group by higher ,middle and lower dose were perfused with yijingfang once a day for fourty-eight days, the higher dose is 3.4g/100g/d, the middle dose is 1.7g/100g/d,the lower dose is 0.85g/100g/d. The normal control group and the model control group perfused with 0.9% NaCl solution of equivalent volume once a day for fourty-eight days. The Gavage capacity IS 1 ml/100g (weight).Both of the volume is 2ml/100g(weight). In the course of experiment, observe the normal conditions of the rats(such as the colour of the fur ,the activity ,the posture and so on) ,measure the volume of water and food the rats had everday. Then all the rats were killed, serum were separated from blood of femoral artery, testis, gonad, and subsidiary gonad were weighted, semen were imbibed from epididymis, Pathological histological examination was performed on the testis. Then we measure the level of gonadal hormone and luteinizing hormone in plasma and the semen density and active ability of epididymis, observe the histological change of testis before and after treatment.Results①Compared to the normal control group, the model control group had significantly lower spermatozoal density, vitality , active rate and the level of testosterone.②Compared to model control group, the higher ,middle and lower dose groups had significantly higher spermatozoal density, vitality, active rate, showed the obvious differences ,but there are no obvious differences among the three treatment groups.③With the treatment of Yijingfang ,the damaged testis and the subsidiary gonad got some extent recovery. Conclusion"Yijingfang", by regulating testosterone (T) secretion and increase the quality of testicular and subsidiary gonads,improve the spermatogenic environment. It can increase the density of sperm and increase the rate of live sperm and vitality,have a good effect on the treatment of the oligospermia and asthenospermia .2.There is no obvious dose-effect relationship between different doses of"Yijingfang"and the improvement of infertility rats leaded by adenine.The lower dose treatment group has more desirable effect.3. the mechanism and action target of Yijingfang treatment in oligospermia and asthenospermia of infertility rats leaded by adenine is still needed for further research.
Keywords/Search Tags:infertility, oligospermia, asthenospermia, yijingfang, adenine
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