Baoshen Tang Experimental Study On The Treatment Of Experimental Rat Renal Interstitial Fibrosis

Purpose: Renal interstitial fibrosis caused by a variety of reasons that the extracellular matrix components in the renal interstitial excessive deposition. Extracellular matrix components including both normal renal interstitial matrix protein components of the increase and normal existence only when the tubular basement membrane matrix protein deposition. Renal interstitial fibrosis almost all kidney disease progress to end-stage renal failure and a common way to the main pathological basis. In recent years, numerous studies show that the degree of tubulointerstitial fibrosis represented the degree of impaired renal function. Therefore, the search for an effective clinical medicine to prevent renal interstitial fibrosis, delaying renal failure is of great significance.Material and method: Use the renal interstitial fibrosis to copy ratmodel, then use the Physical and Chemistry examination method, for example, Optical microscope and so on modern detecting technology, except naked eye observe the renal tissue appearance shape, analysis and validate successful construction of the model, The rats were randomly divided into four groups, namely: the control group, model group, the BAOSHENTANG group, Lotensin group according body weight(200±20g) in fifty Wistar rats of half male and half female. M=14, N=12. The normal group: every day saline 1ml/100g (weight), a lavage, continuous 56 days; Model group: lavage continuous 56 days; The BAOSHENTANG group: After the first 4 week, as the same way every morning, lavage 1ml/100g (weight) every afternoon, continuous 28 days; The piperazine ferulate group: Every morning as the same way, every afternoon to piperazine ferulate 1ml/100g (weight), a lavage, continuous 28 days after the first 4 week. Above all the groups, execute rats at 8:00am after 56 days, when paunch, takeing abdominal aorta blood, kidney, with optical microscope kidney renal tissue pathology, Using automatic biochemical analyzer to measure the level of serum BUN and SCr;Using immunohistochemical methods, semi-quantitative analysis to detect the expression and distribution of HGF, BMP-7, in renal; Using SPSS11.0 software mathematical statistical analysis method.Results:1. Compared with the normal group model group and two treatment groups the levels of blood urea nitrogen significantly increased(P<0. 01); The piperazine ferulate group,BAOSHENTANG group compared with model group the levels of blood urea nitrogen significantly reduced (P < 0. 01); The piperazine ferulate group and BAOSHENTANG group no significant differences (P > 0.05).2. Compared with the normal group model group, the levels of two treatment groups serum significantly increased (P < 0.01); The piperazine ferulate group,BAOSHENTANG group compared with model group the levels of serum significantly reduced (P < 0.01); The piperazine ferulate group and BAOSHENTANG group no significant differences (P > 0.05).3. Compared with the normal group model group, and two treatment groups HGF were significantly increased (P < 0. 01 or P < 0. 05);Compared with the model group , two treatment groups expressed significantly increased (P < 0.01). Comparison between the two treatment groups no difference(P > 0.05). In the groups were compared before and after their two points, the normal group had no significant difference (P > 0.05), model and two treatment groups were significantly lower (P < 0.01).4. Compared with the normal group , model groupand two treatment groups kidney BMP-7 relative content was significantly lower expression (P < 0. 01); Compared with the model group, two treatment groups expressed significantly increased (P < 0.01). Comparison between the two treatment groups no difference(P > 0.05).5. Unaided eye and Macroscopic observation kidney tissues and the group has different levels of renal fibrosis performance. Renal fibrosis each model rats, kidney pale tan positive, to express the model group heaviest. Conclusion:1. BAOSHENTANG can significantly improve pathological injury of rats, with the protection and restoration of renal tissue, delaying the process of renal interstitial fibrosis.2. BAOSHENTANG can reduce the level of serum BUN and SCr obviously.3. BAOSHENTANG can enhance expression and distribution of HGF in renal interstitial fibrosis rat, delaying the process of renal interstitial fibrosis.4. BAOSHENTANG can enhance expression and distribution of BMP7 in renal interstitial fibrosis rat, delaying the process of renal interstitial fibrosis.