Alternanthera Philoxeroides Effective Fractions Of Anti-viral Extract Preparation Process And Its Component Analysis

Altemanthera philoxeroides (Mart.) Griseb., has been used for the treatment of acute brain fever, measles and herpes zoster as a folk medicinal herb. Pharmacological studies indicated that the extract of A. philoxeroides has antivirus,antibacterial and molluscicidal activities.Based on its pharmacology and clinical reseach, the results show that the medicine phenolic acids (including Flavonoids), alkaloids and saponins are anti-viral substances parts. Altemanthera philoxeroides and its injection has received are set out in the1977edition of Chinese Pharmacopoeia, for the treatment of influenza, epidemic hemorrhagic fever caused by a virus disease This paper established a method for the determination of Altemanthera philoxeroides total flavonoids and total saponins. Altemanthera philoxeroides antiviral effective fractions of the extract extraction and purification process were studied, and extract the chemical composition of the various parts of the fingerprint of the foundation for the development of Altemanthera philoxeroides. The effective material fractions extract saponins average content of17.13%, the transfer rate of57.55%; the flavonoids average conten of4.43%transfer rate of64.37%.Using the extracted method of re flowing ethanol and D101macroporous resin purification technology to extract, separate and purify the active site of Altemanthera philoxeroides (Mart.) Griseb., which obtain a reliable technical parameters. Carried out the verifying will be a transition to industrialization.Using HPLC, established the fingerprint of Alternanthera philoxeroides antiviral fractions of phenolic acids, alkaloids and saponins class material.The analytical method has good credibility and stability. The method is stable, well repeated. Fingerprints of10batches of samples of the various types of substances, the phenolic acids on the fingerprints have18common peaks in the fingerprint,the alkaloid substances have18common peaks, saponin substances fingerprint have15common peaks. With reference to the pre-study of the laboratory of Alternanthera philoxeroides anti-virus isolation and identification of the active ingredient, all effective parts of the fingerprint analysis showed that the effective fractions of extracts of Alternanthera philoxeroides contains phenolic acids, alkaloids, saponins, substances active ingredients. Effective parts of the fingerprint of the phenolic acids identified daidzein and oleanolic acid fingerprint peaks, which provide a scientific basis for the interpretation of the material basis of the traditional Chinese medicine.