The Synergistic Effect Of Administration Of Glutamine And Growth Hormone On The Intestinal Barrier Function Of Rats With Obstructive Jaundice

Objective:To evaluate the synergistic effect of the combined use of glutamine and growthhormone on the intestinal barrier function of rats with obstructive jaundice.Method:the study was designed as a prospective, randomized and controlled animalexperiment. The common bile duct was ligated to obtain obstructive jaundice model.Sixty Sprague-Dawley rats were randomly assigned into6groups of10each: GroupⅠ, Control Group; Group Ⅱ, Sham-operated Group; Group Ⅲ,OJ Group; GroupⅣ OJ+Glutamine Group; GroupⅤ,OJ+Growth Hormone Group; GroupⅥ,OJ+Glutamine+Growth Hormone Group. Rats were gavaged with normal saline(1.5ml/d) and glutamine (0.2g/kg/d) in group Ⅰ, Ⅱ, Ⅲ and group Ⅳ, Ⅵrespectively,while rats in group Ⅴ, Ⅵ were treated with growth hormone(2IU/kg/d) by subcutaneous injection once daily for10days.On day9postoperatively，all rats were gavaged with dual saccharides.On postoperative10day,24h urine samples, blood samples in portal vein and abdominal aorta,andblood samples in the inferior caval were collected for L/M tests,endotoxinmeasurements and liver function tests respectively,while liver, spleen,mesentericlymph node(MLN) and terminal ileum samples of rats were obtained for bacterialcultures and morphometry respectively.Results: There were no significantchanges in the L/M value, endotoxin values in portal vein and abdominal aorta, villus height and mucosal thickness of terminal ileum between group Ⅰ and Ⅱ(P>0.05),meanwhile in group Ⅳ they were similar to those in group Ⅴ (P>0.05),however the other results which compared with each other in six groups showedsignificant differences（P<0.05）.The bacterial translocation ratio in group VI wassignificantly lower than that in group Ⅳ or Ⅴ (P<0.0033respectively) whereasthere was no significant difference in that between group Ⅳ and Ⅴ(P>0.0033).The bacterial translocation rate in group Ⅳ was significantly lower than that ingroup Ⅲ(P<0.0033)；The bacterial translocation ratio in group Ⅵ was significantlyhigher than that in group Ⅰor Ⅱ(P<0.0033respectively) while it was similarbetween group Ⅰ and Ⅱ (P>0.0033).Conclusion:The combined administration of GLN and GH in protecting the intestinal barrierfunction of rats with obstructive jaundice is more effective than using each of bothagents alone.