Based On Molecular Markers Assisted Breeding For Ganoderma Lucidum

Ganoderma lucidum is our traditional medicinal fungi which well-known for its role in increasing wisdom and longevity. With the development of large-scale breeding and expansion in cultivation industry of G. lucidum, it is making new demands on the genetics and breeding study. By modern molecular marker technology-assisted will be of great importance in the genetics and breeding study of G. lucidum.This paper mainly does a research on the study of genetic breeding of G.lucidum by CMC test screening 3 of 127 strains from different regions, protoplasted monokaryon preparation, matting type test, the ISSR kinship analysis, the RAPD kinship analysis, the RAPD kinship analysis, genetic relationship analysis, crossbreeding, biological character correlation analyzing technology. The following are research results:1. Three strains:G0119, G0130 and G0154 respectively had been collected from the CMC screening model, the model can screen target G.lucidum strain which was good at dissolving cellulose. Compared with other G.lucidum strains, G0119 and G0154 had a better ability in dissolving cellulose, in contrast, G0130 was not. Meanwhile, the results of the CMC screening model had enriched and extended the genetic resources center of G.lucidum.2.109 monokaryons had been exploited from Glucidum by protoplast monokaryonization, of which 34 from G0119,36 from G0130 and 39 from G0154 respectively. In addition, The relationship among the rate of monokaryotation and growing days showed that the longer growing days, the upper rate of monokaryotation. The results of the matting type test showed that monokaryons with different matting type had obvious morphological variation.3. The genetic diversity of 109 monokaryons of Glucidum were analyzed by ISSR, RAPD, and SRAP molecular markers. The UPGMA dendrogram of monokaryons were based on results of the three molecular markers. The results told that, even though monokaryons were developed from the same starting strain, the disparity among monokaryons also existed. Further more, comparing with using single markers, comprehensive utilization of ISSR, RAPD, and SRAP molecular markers were more effectively and accurately.4. The hybridization of monokaryons generated a total of 63 hybrids with an acquisiton rate of 0.80%. In accordance with the difference of parents which hybridized hybrids, it could be divided into three series called G, T and Z. After verification of antagonistic reaction test,63 hybrids were all new strains.5. The genetic diversity of hybrids of Glucidum was analyzed by ISSR, RAPD, and SRAP molecular markers. The UPGMA dendrogram of hybrids based on results of three molecular markers. The results told that, the genetic diversity among hybrids were abundant, it appeared to be almost no two hybrids were clustered together entirely. The hybids of G, T and Z can be separated orderly.6. Starting with the genetic similarity (GS), we analyzed the GS disparity between the hybrid and the monokaryon. What’s more, the biological character correlation analysis of hybids of biomass, growth rate, polysaccharide, protein content and cellulase activity had been studied. The results told that, the formation of genetic diversity in hybrids because of the variation in the parent monokaryons. In addition, the higher GS of two hybrids, the relationship of biomass, growth rate and cellulase activity in these hybrids were closer, otherwise, far away. Whereas, there was no apparent correlation with GS and polysaccharide and protein content.7. The CMCase relative activity of hybids was no less than the parent strains, it showed that the hybid stain which was based on molecular markersa assisted breeding for was not only no less the parent strains, but also had higher biological efficiency. What is more, The GS value can also study the yield change of biomass and growth rate. Through screening the low GS value between two hybids, it can determine the difference of biomass and growth rate rapidly, then reducing the redundancy of the parent selection. With the judgement of GS, it was conducive to realize the genetic distance of strains, simplify the process of breeding, and reduce the blindness of breeding. Meanwhile, with the results of cultivated fruiting test, some of 63 hybrids could not only be regarded as the commercial variety of factory-like production, but also enriched and extended large-scale breeding, expansion in cultivation industry and the genetic resources center of G.lucidum.