Protective Effects Of Baicalin On Intestinal Mucosa Injury In Immature Rats With Obstructive Jaundice

Objective To investigate the effects of baicalin on intestinal mucosa injury in immature rats with obstructive jaundice (OJ).Methods Forty immature male Wistar rats were randomly divided into four groups: sham operation, OJ, OJ1and OJ2. Rats in groups OJ, OJ1and OJ2were subjected to partial common bile duct ligation, while in sham operation group, bile duct was mobilized but not tied. Baicalin (80mg/kg per day) was fed in groups OJ1and OJ2, while in the other groups, normal saline was fed. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) total bilirubin (TBIL), and direct bilirubin (DBIL), were obseved by automatic biochemical analyzer.Ileum mucosa were prepared for light microscopic examination. Expression levels of NF-κBp65mRNA, NF-KBp65protein, MMP-9and apoptosis indexes in intestinal mucosa were determined.Results (1) There was no significant difference among all groups of ALP,TBIL,and DBIL. ALT and AST level in OJ group is significantly higher than that of the other groups,P<0.01.(2) When compared to OJ group (3.2±0.5), the pathological severity scores of intestinal mucosa significantly declined (P<0.01) in groups sham operation (0.4±0.3),OJ1(1.9±0.2) and OJ2(1.5±0.3);(2) Rats in OJ group presented significantly upregulated the expression of NF-κBp65and MMP-9in intestinal mucosa, when compared with sham operation group, OJ1group and OJ2group, P<0.01.(3) The apoptosis indexes significantly decreased in sham group (7.5±1.6), OJ1group (15.1±1.6) and OJ2group (14.3±2.2)(P<0.01),when compared with OJ group (27.6±2.1).(4) When compared to OJ group, the positive expression rates of NF-κBp65mRNA of intestinal mucosal epithelium in the other groups significantly decreased.Conclusion The result showed that baicalin inhibits the transcription and translation of NF-κBp65, down-regulates MMp-9protein expression, reduces apoptosis of intestinal mucosa in immature rats with OJ. It is suggested that baicalin exert protective effects on the intestinal mucosa injury in immature rats with OJ.