Expression Of VDR And IAP In Intestinal Mucosa Of Patients With Obstructive Jaundice

Objective:Obstructive jaundice can cause damage to the intestinal mucosal barrier,while VDR(vitamin D receptor)and IAP(intestinal alkaline phosphatase)play an important role in maintaining intestinal mucosal function,but the changes between damage and VDR and IAP have not been studied.in the intestinal mucosal barrier in patients with obstructive jaundice.Therefore,the purpose of this study was to investigate the difference in expression of VDR and IAP in intestinal mucosa between patients with obstructive jaundice and non-jaundice.Methods:Twenty-three patients with pancreaticoduodenectomy,hilar cholangiocarcinoma or biliary anastomosis were enrolled in the Shengjing Hospital affiliated to China Medical University,and these patients were enrolled as a jaundice group.A total of 10 patients which were non jaundice were collected as a non jaundice group.All patients underwent jejunal tissue in the specimen after surgical resection.It has been approved by the Ethics Committee before collecting the specimen.Part of the collected jejunal tissue was used to observe intestinal mucosal morphology and immunohistochemistry experiments;the other part was used for Western-blot detection to determine the expression of VDR and IAP.The data obtained were statistically analyzed using Graphpad prism 6 analysis software,and the differences in data distribution between the two groups were analyzed by Mann-whitney U test;P < 0.05 was considered statistically significant.Results:The intestinal mucosal barrier of patients with obstructive jaundice was significantly impaired under the light microscope,the mucosal length became shorter,and the mucosa was sparse.The morphology of intestinal mucosa changed significantly,and some of them showed lodging.While there was no significant change in intestinal mucosal barrier in patients with non jaundice.Immunohistochemical results: Microscopically,compared with the non jaundice group,the proportion of cells stained with VDR primary antibody was significantly decreased in the obstructive group,it means the expression level of VDR in the obstructive group is lower than that in the non jaundice group,the difference is statistically significant(Mann-Whitney U=44.5,P=0.0160).And the expression level of IAP in the obstructive group is lower than that in the non jaundice group,too.The difference was statistically significant(Mann-Whitney U=40,P=0.0087).Western-blot results: The VDR/GAPDH ratio of the non jaundice group was greater than that of the corresponding obstructive group,suggesting that the expression level of VDR in the non jaundice group was higher than that in the obstructive group.The difference was statistically significant(Mann-Whitney U=41,P=0.0104).At the same time,the IAP/GAPDH ratio of the non jaundice group was greater than that of the corresponding obstructive group,suggesting that the expression level of IAP in the non jaundice group was higher than that in the obstructive group.The difference was statistically significant(Mann-Whitney U=45,P=0.0178).Conclusion:The intestinal mucosal barrier of patients with obstructive jaundice was significantly impaired.The expression of VDR in intestinal mucosa of patients with obstructive jaundice was lower than that of patients with non jaundice.The expression of IAP in intestinal mucosa of patients with obstructive jaundice was lower than that of patients with non jaundice.