The Study Of Effects Of IL-6on The Expressions Of Bcl-2, CyclinD1and VEGF In Ovarian Cancer Cells Via STAT3Pathway

ObjectiveTo investigate the relationships between IL-6and protein expression ofBcl-2, CyclinD1, VEGF and the STAT3pathway in epithelial ovarian cancerSKOV3.Methods1、SKOV3cells were treated for24and48hour with diverse concentrationsof IL-6（0,10,20,40ng/ml）respectively. The proliferation and apoptosis of cellswere detected by MTT and flow cytometry. The mRNA expression of Bcl-2,CyclinD1and VEGF in cells was determined by RT-PCR. The proteinexpression of Bcl-2, CyclinD1, VEGF and p-STAT3in cells was measured bywestern blotting.2、SKOV3cell were treated, in the presence of optimal concentrations ofIL-6（20ng/ml）, with diverse concentrations of AG490（0,20,40,80,160μmol/l）,a specific JAK kinase inhibitor, respectively. The proliferation and apoptosis ofcells, the mRNA and protein expression of Bcl-2, CyclinD1, VEGF and p-STAT3was remeasured, as previously described.3、The results were expressed as mean±standard error of the mean（SEM）. SPSS16.0was involved and statistical analyses were performed byT-test for paired samples comparisons, ANOVA for multiple comparisons.Significance was assumed at P＜0.05.Results1、With the increase of IL-6concentration（10,20ng/ml）, the action of promoting proliferation（P＜0.01）and inhibiting apoptosis（P＜0.01）wereenhanced, the mRNA and protein expression of Bcl-2, CyclinD1, VEGF andp-STAT3were increased（P＜0.01）, compared to the control group. Furthermore, the peak action was obtained at the concentration of20ng/ml. There weredifferences between48h and24h（P＜0.01）. Compared with IL-6groups of20ng/ml, the group of40ng/ml is no significance（P＞0.05）.2、With the increase of AG490concentration（20,40,80μmol/l）, the actionof inhibiting proliferation（P＜0.01）and promoting apoptosis（P＜0.01）wereenhanced, and the mRNA and protein expression of Bcl-2, CyclinD1, VEGF andp-STAT3were decreased（P＜0.01）, compared to the IL-6control group.Further more, the peak action was obtained at the concentration of80μmol/l.Compared with AG490groups of80μmol/l, the group of160μmol/l is nosignificance（P＞0.05）.ConclusionIL-6promotes the gene transcription and expression of CyclinD1, VEGFand Bcl-2, contributing to the proliferation of ovarian cancer and inhibiting itsapoptosis may via STAT3pathway.