A Study Of The Relationship Between Kidney-Yang Deficiency Rats Induced By Adenine And Serum Steroid Hormones

Objective Observing the changes in serum steroid hormones of kidney-Yang deficiency rats induced by adenine,and jinkuishenqi pills’ intervention effect of kidney-Yang deficiency. Preliminary discussing the relationship between the rat kidney-Yang deficiency and Serum steroid hormones.Methods1.42healthy male SD rats,weight180-220g.After normal breeding for seven days,according to random number table randomly selected16rats,giving normal saline breeding. The remaining26given adenine mold processing for30consecutive days. The adenine30mg per l00g body weight of rats with distilled water configured into2ml suspension.2.Buliding after30days,respectively from the normal group and the module randomly selected6rats,put to death and took blood,determined the content of testosterone(T)、cortisol and adrenocorticotropic hormone(ACTH) to determine whether the rat building succeed.3After the success of the building is confirmed,model group were randomly divided into model control group and jinkuishenqi pills treatment group, coupled with the normal control group,there was a total of three groups and each group of10.4.Model control group and normal control group rats were given2ml saline lavage every day,and the jinkuishenqi pills treatment group was0.20g/100g/d configured lavage2ml suspension with distilled water on30consecutive days.5.Record the change of the rat body weight per week, Observe the life of rats every day such as general situation. Among them,there are two rats died on the experimental process,model control group and jinkuishenqi pills treatment group respectively one. After30days’ medicine delivery,fasting for24h and put to death all the rats. Took blood from abdominal aorta and dissected to take kindey tissuse.6.With HE staining,observed the damage of renal tissue under the electron microscope;determined the value of serum urea nitrogen and creatinine;using HPLC/MS/MS detected the content of serum steroid hormones(testosterone,progesterone,17alpha hydroxyprogesterone,11-deoxidization cortisol, corticosterone, androstenedione, deoxidization corticosterone,pregnenolone, cortisol).Results1.Compared with normal group,①testosterone,17alpha hydroxy progesterone, androstenedione, corticosterone and cortisol appear the phenomenon of significantly lower(P<0.01or P<0.05),among them, the reduce of testosterone and androstenedione were most obvious.②ogesterone,11-deoxidization cortisol,11-desoxycorticosterone, pregnenolone significantly elevated(P<0.01),among them, the elevate of pregnenolone were most obvious.2.Compared with model group.jinkuishenqi pills can significantly improve testosterone,17alpha hydroxyprogesterone, androstenedione, corticosterone and cortisol levels and significantly lower progesterone,11-deoxidization cortisol,11-deoxidization of corticosterone,pregnenolone hormone levels(P<0.01),to make it close to normal.Conclusion1.The kidney-yang deficiency rats induced by adenine is significantly related to the metabolic disorders of serum steroid hormones.2. Jinkuishenqi pills can significantly improve the metabolic disorders of Serum steroid hormones induced by kidney-yang deficiency.