The Effect Of Tortoise Shell Gum On The Antioxidant Activity And The Expression Of Bax And Bcl-2 Protein In Rats With Kidney Yin Deficiency

Objective: to observe the effects of tortoiseshell gum on organ index(liver,kidney),body mass,anal temperature,water consumption and food intake in rats with kidney-yin deficiency.To investigate the effects of tortoise shell gum on SOD activity,MDA content,gsh-px,E2,T level and Bax and bcl-2 protein expression in renal tissues of rats with kidney Yin deficiency.Methods:after 3 days of adaptive feeding,60 SPF female SD rats were randomly divided into normal group,model group,drug administration group(low dose group,medium dose group,high dose group)and positive group(liuwei dihuang pill)for 21 days.Except the normal group,the pathological model of kidney-yin deficiency was established by gavage of thyroxine.The normal group and the model group were given the same volume of normal saline every day.During administration,the changes of viscera(liver and kidney)index,body mass,anal temperature,water consumption and food intake were observed.Serum levels of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),estradiol(E2)and testosterone(T)were detected by enzyme-linked immunosorbent assay(ELISA).The expression of Bax and bcl-2 was detected by immunohistochemistry.RT-q PCR was used to detect the relative m RNA expression of Bax and bcl-2 proteins,and the results were compared between groups.Results:the condition of Yin deficiency of rats was significantly alleviated in all the treatment groups,the body mass and liver index of rats with kidney Yin deficiency were significantly increased in the medium and high dose groups(P<0.05,P<0.01),and the kidney index of rats with kidney Yin deficiency was significantly increased in the high dose group(P<0.01).The anal temperature of rats with kidney-yin deficiency was decreased in all dose groups(P<0.05,P<0.01).The levels of SOD,GSH-Px and E2 in the serum of rats in the medium and high dose groups increased(P<0.05,P<0.01),while the levels of MDA decreased(P<0.05).The serum T content of rats in the high dose group was significantly increased(P<0.05,P<0.01).Bax expression level in the kidney of rats in the low and high dose groups was decreased(P<0.05).The expression level of bcl-2 in the kidney of rats in the high dose group was significantly increased(P<0.01).Conclusion:(1)The pathological model of kidney-yin deficiency rats was successfully produced by gavage of thyroxine.To some extent,tortoise shell gum can alleviate the pathological changes of kidney tissue in rats with kidney-yin deficiency.(2)The body mass and liver index of rats with kidney-yin deficiency were significantly increased in both the medium and high dose groups,while the kidney index of rats with kidney-yin deficiency was significantly increased in the high dose group.The anal temperature of rats with kidney-yin deficiency was decreased in the low,medium and high groups.(3)The contents of SOD,gsh-px and E2 in the serum of rats in the medium and high dose groups increased,while the contents of MDA decreased.The serum T content of rats in the high dose group was significantly increased.It was suggested that tortoise shell gum could improve the antioxidant capacity of rats,and had certain pharmacological effect to improve the syndrome of kidney Yin deficiency in rats,but the effect of lower dose tortoise shell gum was not significant.(4)Bax expression level in the kidney tissues of rats in the low and high dose groups was decreased;The expression level of bcl-2 in the kidney of rats in the high dose group was significantly increased.It was suggested that high dose of tortoise shell gum could down-regulate Bax,up-regulate the expression of bcl-2,inhibit the apoptosis of renal tubular epithelial cells,and the effect was slightly better than that of liuwei dihuang pill.