Study On Medication Rule And Network Pharmacology Of Core Formula Of The Method Of Toniftying Kidneyand Removing Blood Stasis In The Treatment Of Chronic Kidney Disease

Objective: Based on literature reports,to explore the potential medication rule of method of tonifying kidney and removing blood stasis in the treatment of chronic kidney disease,and to explore its possible mechanism of action.Method:1.Search CNKI,Wanfang Database and VIP database with the main titles of "Tonifying kidney","removing blood stasis" and "chronic kidney disease",screen the literature according to the inclusion and exclusion criteria,and import the data into the TCMSP V3.0 after standardized drug names processing.The corresponding sections were selected for drug frequency,drug category,four qi,five flavor,and meridian frequency statistics.After setting confidence and support,association rule results were obtained.k-means clustering algorithm was used to obtain cluster analysis results,and the core prescription of tonifying kidney and removing blood stasis method for the treatment of chronic kidney disease was obtained through comprehensive analysis.2.The drugs contained in the core prescriptions were successively imported into the TSMSP database for active ingredient search and target prediction,and disease targets related to "chronic kidney disease" were searched in Genecards,TTD,OMIM,Pharm Gkb and Drug Bank databases for screening and combination,and the intersection of drugs and disease targets was obtained using R software.The core-component-target-chronic renal disease regulatory network was constructed using Cytoscape3.9.1,the protein interaction relationship was obtained by STRING database,and the protein interaction network was constructed.Then,GO and KEGG enrichment analysis was performed using R software.Results:1.According to inclusion and exclusion criteria,176 effective literatures were obtained,and 261 drugs were used.2.The statistics of drug frequency showed that the top 6 herbs were Huangqi(82.4%),Danshen(68.2%),Fuling(45.5%),Chuanxiong(44.9%),Shanyao(43.2%)and Shanzhuyu(42.0%).The main drug efficacy categories were deficiency supplementing(38.4%)and blood circulation removing blood stasis(22.4%).The four gas analysis results from high to low were as follows: warm(38.9%),flat(31.1%),cold(27.1%),hot(2.2%),cool(0.6%).The five taste statistics showed that sweet(42.8%),bitter(28.6%),spicy(17.3%),sour(7.3%)and salty(4.0%)in descending order.The meridian frequency statistical results were liver channel(22.7%),spleen channel(18.2%),kidney channel(16.4%).3.Association rule results showed that the top three core drug combinations were Huangqi-Danshen(102 times),Huangqi-Fuling(70 times),Huangqi-Shanyao(66 times),Huangqi-Chuanxiong(62 times),and Huangqi-Shanzhuyu(62 times).4.Five core prescriptions were obtained by cluster analysis.According to the results of association rules and cluster analysis,the core prescription components were obtained:Huangqi,Danshen,Fuling,Chuanxiong,Shanyao and Shanzhuyu.5.Network pharmacological research results showed that quercetin,luteolin,kaempferol,tanshinone ⅡA and cryptotanshinone were the main core active ingredients in the treatment of CKD by tonifying kidney and removing blood stasis.The core targets of regulation are TNF,CASP3,IL10,IL6,IL1 B,CXCL8,STAT3,PTGS2,TP53,MMP9,VEGFA,HIF1 A,CCL2 and EGF.It mainly plays a role in pathway signaling of AGEs-RAGE,fluid shear stress and atherosclerosis,lipid and atherosclerosis,PI3K/AKT,TNF,HIF1,IL-17,multiple tumors,and multiple viral infections.Conclusion:1.The main drugs used in the treatment of CKD by the method of tonifying kidney and removing blood stasis are sweet and warm,mainly restoring liver,spleen and kidney channels,and the most common drugs are tonifying deficiency and promoting blood circulation and removing blood stasis.The core prescriptions include Huangqi,Danshen,Fuling,Chuanxiong,Shanyao and Shanzhuyu.2.The method of tonifying kidney and removing blood stasis Core formula may regulate the expression of PTGS2,TNF,IL-6,IL-10,STAT3,MMP9,CXCL8,TP53 and other cytokines through quercetin,luteolin,kaempferol,tanshinone,cryptotanshinone and other active ingredients.Regulation of AGEs-RAGE,fluid shear stress and atherosclerosis,lipid and atherosclerosis,PI3K/AKT signaling pathways play anti-inflammatory,antioxidant stress,anti-atherosclerosis and anti-fibrosis pathways,and delay the progression of CKD.