Data Mining And Network Pharmacology Of Traditional Chinese Medicine In The Treatment Of Ulcerative Colitis With Damp-heat

Objective:The data mining method was used to collect and sort out the relevant literature of oral traditional Chinese medicine for the treatment of ulcerative colitis with damp-heat,and obtain its medication rules and core traditional Chinese medicines.The mechanism of the treatment of ulcerative colitis with damp-heat was obtained by using the network pharmacology research Institute.Therefore,the medication rules and clinical experience of clinicians in the diagnosis and treatment of ulcerative colitis with damp-heat are summarized.Method:1.Randomized controlled trials on oral Chinese medicine in the treatment of ulcerative colitis with damp-heat have been published on CNKI,Wanfang,VIP,Pub Med and other databases.The data is normalized and the database is established.Through frequency analysis,association rules,cluster analysis and other technologies,statistical software is used to mine data for prescription drugs.2.Through network pharmacological analysis,it were screened that the active ingredients and target proteins of the core Chinese medicines for the treatment of ulcerative colitis with damp-heat in TCMSP and ETCM databases.For the active constituents without relevant target protein information in TCMSP database,Pubchem and Swiss Target Prediction platform was used as a supplement.Then the Dis Ge Net,Drug Bank,OMIM,TTD,and Gene Cards databases were used to search and screen ulcerative colitis disease targets.The intersection of the two targets is visualized and analyzed through the Venny platform.Cytoscape 3.9.0 software is used to construct a "drug-target-disease" network viewable.And PPI network can be viewed through STRING database architecture.Finally,the Metascape database was used for GO enrichment analysis and KEGG signaling pathway analysis.Result:1.In this study,138 articles were included,of which the most evidence of large intestine damp-heat was 57 times.152 flavors of traditional Chinese medicine are involved.The top 10 most frequently used Chinese medicines were Rhizoma Coptidis,licorice root,Radix Aucklandiae,Radix Paeoniae Alba,Radix Pulsatillae,Rhizoma Atractylodis Macrocephalae,Radix Scutellariae,Radix Angelicae Sinensis,Poria,Cortex Phellodendri Chinensis.In terms of efficacy,the Chinese medicines involve 38 categories,and the Chinese medicines with the highest frequency of use are clear heat and dry dampness medicine,supplement qi medicine,clear heat and resolve toxins medicine,rectify qi medicine,and hydrating and detumescent medicine.The frequency sequence of the four qi is cold,warm,neutral,cool and hot in traditional Chinese medicine.The ranking of the five flavors is,in order bitter,sweet,acrid,sour,astringent,bland,salty.The top four are spleen channel,stomach channel,liver channel and large intestine channel in the channel entry ranking.2.44 core drug pairs were obtained by association rule analysis of drugs used in ulcerative colitis with damp-heat.Common drug pairs include:Rhizoma Coptidis,Cortex Cinnamomi;Radix et Rhizoma Glycyrrhizae,Radix Codonopsis,Rhizoma Coptidis;Rhizoma Coptidis,Radix Scutellariae,Radix Aucklandiae;Rhizoma Coptidis,Radix Angelicae Sinensis,Radix Aucklandiae and so on.3.Medication cluster analysis of ulcerative colitis with damp-heat yielded 9 clustering results:C1: Rhizoma Coptidis,Radix Aucklandiae,Semen Arecae,Cortex Cinnamomi,Radix Angelicae Sinensis,Radix Paeoniae Alba;C2: Radix et Rhizoma Glycyrrhizae,Radix Scutellariae;C3:Radix Sanguisorbae,Herba Agrimoniae,sanchi;C4: Radix Puerariae Lobatae,Rhizoma Bletillae;C5: Herba Portulacae,purslane,citrus;C6: Semen Coicis,patrinia;C7:Poria,Radix Codonopsis,Rhizoma Atractylodis Macrocephalae,Radix Astragali;C8:Cortex Phellodendri Chinensis,Cortex Fraxini,Radix Pulsatillae.4.A total of 53 active ingredients,795 target proteins,744 disease targets and 147 intersection targets are obtained by network pharmacological analysis.The main active ingredients include quercetin,kaempferol,oleic acid,linoleic acid,parmatine,berberine,etc.The core targets of protein interaction network analysis include STAT3,PIK3 CA,RELA,IL6,PTPN11,TNF,STAT1,IL10,KRAS,JAK2,etc.GO analysis yielded a total of 5357 entries related to UC bioprocesses in core Chinese medicine treatment.There are 827 entries related to molecular function.There are 436 entries related to cell composition.KEGG signal path analysis yielded 191 related signal paths.It mainly includes cancer pathway,PI3K-Akt signaling pathway,TNF signaling pathway,JAK-STAT signaling pathway,NF-κB signaling pathway,chemokine signaling pathway,inflammatory bowel disease and other signaling pathways.Conclusion:1.Data mining studies have shown that ulcerative colitis with damp-heat is located in the large intestine and is closely related to the spleen,stomach and liver.The core traditional Chinese medicine(Shaoyao Decoction),which has the function of clear heat and dry dampness and regulate and harmonize qi and blood,is a common prescription for the treatment of ulcerative colitis with damp-heat.2.These may play a role in inhibiting inflammatory response,oxidative stress,regulating intestinal microorganisms,inhibiting apoptosis,protecting the intestinal mucosal barrier and other functions through cancer,PI3K-Akt,TNF,JAK-STAT,NF-κB and other signaling pathways,so as to achieve the effect of treating ulcerative colitis with damp-heat.