Microbialtransformation Of Rhubarb Free Anthraquinones And Bufodienolides

Microbialtransformation was employed to modify the structures of rhubarb free anthraquinones and toad venum bufodienolides. In the present study, twenty-five microbial strains were screened for the ability to transform chrysophanol, physcion, emodin and aloe-emodin. Absidia coerulea (3.3389), Absidia coerulea (3.3382) were found able to transform chrysophanol, physcion, emodin and aloe-emodin. Mucor spinosus (3.3450), and Mucor spinosus (3.2450) were able to transform chrysophanol, physcion and emodin.Absidia coerulea (3.3389) was employed for the biotransformation of chrysophanol, physcion, emodin and aloe-emodin. Four metabolites were obtained. On the basis of their physio-chemical data, the structures of the biotransformed products were characterized as chrysophanol-1-O-β-D-glucop-yranoside, physcion-8-O-β-D-glucopyranoside, emodin-6-O-β-D-glucopyranosi de, and aloe-emodin-1-O-β-D-glucopyranoside respectively.Mucor spinosus (3.3450) was employed for the biotransformation of chrysophanol, physcion and emodin. Four metabolites were obtained. On the basis of their physio-chemical data, the structures of the biotransformed products were identified as chrysophanol-1-O-β-D-glucopyranoside, physcion-1 -O-β-D-glucopyran-oside, physcion-8-O-β-D-glucopyranoside and citreorosein respectively.When chrysophanol was incubated with Mucor spinosus culture inwhich galactose was added to potato medium as carbohydrate instead of glucose, it was found that biotransformed product was the mixture of chrysophanol-1-O-β-D-glucopyranoside and chrysophanol-8-O-β-D-glucopyranoside. By this study, we obtained the preliminary data regarding the influence of different lands of carbohydrate in potato medium on the glycosylation.Mucor spinosus (3.3450) was employed for the biotransformation ofcinobufagin, the biotransformated products were identified as 1β-hydroxy-cinobufagin, 12p-hydroxy-cinobufagin and 1β, 12β-dihydroxy-cinobufagin respectively.Alternaria alternat (3.577) was employed for the biotransformation of cinobufagin, the biotransfornied product was 3-oxo, 12p-hydroxy-cinobufagin. Among the transformed products, 1β-hydroxy-cinobufagin, 1β, 12β-dihydroxy-cinobufagin and 3-oxo, 12β-hydroxy-cinobufagin are new compounds.Cunninghamella blakesleana (3.970) was employed for the biotransformation of marinobufagin, the biotransformed product was 16β-hydroxy-mainobufagin.Cytotoxic activities of 1β-hydroxy-cinobufagin, 12β-hydroxy-cinobufagin, 1β, 12β-dihydroxy-cinobufagih, 3-oxo, 12β-hydroxy-cinobufagin and 16β-hydroxy-marinobufagin against the tumor cells of Bel 7402, BGC 823, HeLa, and HL60 were screened in vitro by MTT method, among which 1β -hydroxy-cinobufagin, 12β-hydroxy-cinobufagin, 1β, 12β-dihydroxy-cinobufa-gin and 3-oxo, 12β-hydroxy-cinobufagin showed good inhibitive activity.