Study On Polysaccharides From Fructus Corni And Separation Of Flavones And Tea Polyphenol With Macroreticular Resins

There are abundant herbal medicines in China, which has great potential to be developed. Polysaccharide is the basis for its biological activity and pharmacology functions such as anti-oxidation and anticancer. In chapter 1 to chapter 4 of this dissertation, separation, characteristics and biological activities of polysaccharides from Fructus corni were investigated. Flavones is another important effective components in herbal medicines, which is often separated with macroreticular resins. In chapter 5 of this dissertation, separation process of total flavones of Hippophae and tea polyphenols with macroreticular resins were studied, and a method of choosing desorption solvent using solubility parameters was put forward theoretically and experimentally.(1) Several factors that affecting the extraction process were studied through single factor and orthogonal experiments. The optimal technological conditions of water extraction from Fructus corni were decided: the temperature being 80°C, the extraction time being 2 hours, the ratio of solid material to water being 1 : 16, the granularity of Fructus corni being 300-450 μm, the times of extraction at the same ratio of solid material to water being 4, and the times of clearing protein being 6. A purified polysaccharide PFCAIII was prepared with process as the following: water extracting → DEAE-cellulose chromatographic fractionating → sephadex chromatographic purifying. Another purified polysaccharide PFCC I was prepared with process as the following: extracting with sodium hydroxide → DEAE-cellulose chromatographic fractionating→ sephadex chromatographic purifying.