Research On Microbial Population Structure On Soil Suitability Of Salvia Miltiorrhiza Origin

Radix Salviae Miltiorrhizae, Chinese herbal medicinal frequently used, was dry radix and rhizoma of Salvia miltiorrhiza Bunge, promoting blood circulation, menstrual pain, pure heart and arrest restless, cooling elimination effectiveness of carbuncle. Continuous cropping of Salvia miltiorrhiza seriously harms to the quality of Radix and Rhizoma Salviae Miltiorrhizae in cultivation process, a threat to the development of its economic, causing huge economic loss. This research article, based on the theory of continuous cropping obstacles, throngh the soil microbial research in the producing Salvia miltiorrhiza regions, evaluated microbial structure characteristic of soil suitability. Results were as follow.The research on soil microbial at the uncropping Salvia miltiorrhiza areas of Zhongjiang county indicated that soil bacteria was increasing, but soil fungi was slow with the years off. Cluster analysis showed that that population of soil microbial mainly changed after continuous uncropping Salvia miltiorrhiza 2 years. The functional microbial population of Zhongjiang county was obviously different among uncropping Salvia miltiorrhiza years. With years off, cellulytic bacteria and aromatic compound decomposed bacteria were significantly increasing while other microbial population tended to decrease. And biodiversity index was increasing. MBP contents were significantly different among years uncropping Salvia miltiorrhiza at Zhongjiang county, and MBP contents obviously changedafter uncropping Salvia miltiorrhiza, which was consistent with the results of culturable microbial. With the years on of uncropping Salvia miltiorrhiza, DGGE bands rechness of bacteria genetic diversity was decreasing, but DGGE bands rechness of fungi genetic diversity was increasing.The number of culturable microbial in the suitably producing Salvia miltiorrhiza areas was significant difference with number of culturable microbial in the insuitably producing Salvia miltiorrhiza areas. The number of fungi at the areas of suitably producing Salvia miltiorrhiza was obviously greater than that of insuitably producing. The numbers of anaerobic cellulytic bacteria, nitrification bacteria and anti-surfur bacteria at the areas of suitably producing Salvia miltiorrhiza were significantly more than that of insuitably producing Salvia miltiorrhiza. Other functional microbial population difference was not to the level of statistical significance, and as to biodiversity index, which was relevant to environmental factors in the regions of producing Salvia miltiorrhiza all over the country. MBP contents at the areas of suitably producing Salvia miltiorrhiza were significantly higher than that at the areas of insuitably producing Salvia miltiorrhiza, which was inconsistent with the research results of culturable microbial population. PCR-DGGE molecular analysis technology can be a good tool to analyse culturable and unculturable microbial community genetic structure, and complement and support the traditional analysis method. Fungi and bacteria genetic diversity cluster analysis showed that soil microbial genetic distance of cropping Salvia miltiorrhiza in 2005 and 2006 was smaller than that of other years, and similar, gathered each other; soil microbial genetic distance of cropping Salvia miltiorrhiza in 2008 and 2009 was smaller than that of 2005 and 2006, and similar, gathered each other, while soil microbial genetic diversity of cropping Salvia miltiorrhiza in 2007 distributed in the two categories, seeming transition between the two categories. This was consistent on the results of culturable microbial research. Soil microbial genetic diversity was complex all over the country. But cluster analysis showed that the genetic distance of the suitably producing Salvia miltiorrhiza areas was relatively smaller than that of others. The genetic distance of the insuitably producing Salvia miltiorrhiza areas was quite different.Sequencing, comparison with the GENEBANK sequence database, the results showed that bacteria was in the genus of alpha proteobacterium, Sphingomonas, Arthrobacter, and fungi was in the genus of Termitaria, Agaricus, Cercozoan, Cordyceps, Geomyces, Penicillium, Uncultured Iodophanus.Functional microbial population of rhizosphere and the chemical components of Salvia miltiorrhiza Bunge for stepwise regression analysis showed that caffeic acid comtent was associated with the population of C circulation, tanshinone IIA content was associated with the population of N circulation, salvianolic acid A was associated with the population of N/P, N/S circulation.It was the first time that microbial community structure associate with active ingredients of Salvia miltirrhiza Bunge.Through LOGIT stepwise regression analysis on the rhizosphere soil microbial and the control soil microbial, results showed that equations were used to determine whether soil suitability for implanting Salvia miltiorrhiza Bunge the first time. Y=e (-1.4560191nyx-0.6342218lnfs-5.300482)-0.4The main factor causing continuous cropping obstacle of Salvia miltiorrhiza was that the soil microbial community was destroyed. And uncontinuous cropping Salvia miltiorrhiza was to rebuild and restore soil microbial population balance. Continuous 2-3 years uncropping Salvia miltiorrhiza was the key period of soil microbial communitiy reconstruction and restoration.