The Influence Of Docomposing Of Fibrous Roots To Allelochemicals And Microbial Flora In Salvia Miltiorrhiza Bge Continuous Cropping Soil

Objective: The residual Salvia fibrous roots after harvest will affect the growth of Salvia, aiming at this problem, added the fibrous roots in the test soil.The yield and contents of active components of Salvia and the physical and chemical properties, allelochemicals, and microbial flora in salvia miltiorrhiza Bge rhizosphere soil were studied under the condition of fibrous roots natural decay. Compared the influence and parameters and determined the main factor. The research will provide the scientific basis for making reduction technology of continuous cropping obstacle.Method: A pot experiments were added different amounts of fibrous roots in the test soil, the treatment were CK, treatment 1(20 g), treatment 2(40 g) respectively. The Salvia were cultivated under the condition of natural decay, The contents of active components were detected by HPLC, nutrients were detected by ICP. allelochemicals were detected by GC-MS, the soil microbial community structure were detected by PCR-DGGE and Miseq.Result: 1. Fibrous roots decay decreased the production of salvia plant by 38.60% and 30.99% for(20 g) and(40 g), respectively. A little amount of fibrous roots decay(20 g) could increased the content of dihydrotanshinone and Cryptotanshinone of Salvia by 26.08% and 22.64%, more fibrous roots decay made the content of ihydrotanshinone, Cryptotanshinone, tanshinone Ⅰand tanshinone ⅡA of Salvia decreased by 60.87%, 79.24%, 84.61% and 88.99% respectively. The different concentration of fibrous roots decay could enhance the content of salvianolic acid B Significantly, and the increased proportion of(20 g) 4.98% and(40 g)23.64%, respectively. meanwhile, the content of Rosemary acid increased by(20 g) 4.98% and(40 g)23.64% respectively.2. The different decomposition degrees of fibrous root decreased the pH and resulted soil acidification,compared with CK decreased 4.5%(20 g)and 4.98%(40 g)respectively. also notably reduced the content of Ca, Fe, K, Zn and N with the number 1.3%, 1.99%, 0.14%, 6.17% and 15.38% for treatment 1 respectively, the number for treatment 2 were 4.28%, 3.75%, 2.47%, 6.17% and 53.84% respectively, meanwhile, decreased the content of Cu, Mg, Mn and P with the number 4.3%, 2.7%, 1.5% and 4.8% for treatment 2.3. Fibrous roots decay could increase the content and species of allelochemicals compared with CK, acetabldehyde, octanoic acid, palmitic acid and icosane appeared in treatment 1, meanwhile, acetabldehyde, octanoic acid, n-hexanol, palmitic and phthalate appeared in treatment 2. The total content of acid components in acid component increased 10.07 flod in treatment 1 and 19.30 flod in treatment 2.4. Fibrous root treatment reduced the number of bacteria species and abundance of proteobacteria and cellvibrio in soil, while the quantity of fungi species, the abundance of helvella, coniosporium were increased and clydaea was added as well. The number of bacteria darmaticlly decreased as the increasing input of fibrous root. Continuous cropping made the number of bacteria increase and genus decrease except cellvibrio. The abundance of unclassified fungi gained and knufia, clydaea, rhizoctonia joined as new members.Conclusion: Fibrous root deacy decreased the pH and resulted soil acidification and supply capacity reduction, meanwhile, it increased the content and species of allelochemicals made the microbial community composition change, that decreased the number of bacterial and increased the number of fungi, also decreased the quality of Salvia, made the continuous cropping obstacle formation.