Studies On The Protective Effect And Mechanism Of Glutamine On Intestines In Early-weaned Piglets

The main objective of this dissertation is to determine the content of glutamine in sow抦ilk and investigate the effect of dietaiy glutamine supplementation on growth performance, intestinal structure and functions and protective mechanism for intestinal injury of piglets. The results show that glutamine was a conditionally essential amino acid for early-weaned piglets. Dietary glutamine supplementation improved growth performance , maintained intestinal normal structure and function. improved antioxidative capacity of piglets. The study provided theoiy basis for dietary glutamine supplementation in early-weaned piglets and they could be saved as an animal model for people nutrition. . The dynamic change in the concentration of conventional nutrients, insulin and epidermal growth factor in colostrum and milk of sows Milks samples were collected from 8 normal 3~-4 years largewhite. Landrace and BeijingBlack lactating sows that had farrowed litters containing 9梋 3 live piglets. Within 35 days the dynamic change in the concentration of conventional nutrients , insulin and EFG in sows colostrum and milk was analyzed, The result indicated the change in the concentration of total solids, protein, insulin and EFG was significant between colostntm and milk. The concentrations of total solids, protein, EFG and insulin were very high in colostrum. .The concentrations of lactose and fat significantly increased by time of lactation ,especially in colostrum. But the change of concentrations of lactose and fat in milk was not significantly. The levels of free glutamine were lower in colostnim than in milk. 2. Effect of dietary glutamine level on growth performance and pancreatic endocrine of early-weaned piglets Seventy-two piglets weaned at the 21 d of age were randomly assigned to one of four dietary treatrnents(0, 0.5, 1.0 and 1.5% glutamine supplementation), there were three replicates in each treatment and six piglets in each replicate. The diets were calculated to be isonitmgenous and isoenergetic . Dietaxy glutamine supplementation improved growth performance and feed conversion rate of piglets , reduced the fall of plasma glutamine level after weaned . The level of plasma insulin significantly increased at the 35d of age and then significantly decreased at the 49d of age, and the response was related to dose. The level of plasma gjucagon not significantly altered. glutamine was a conditionally essential amino acid for early-weaned piglets. Dietary glutamine supplementation improved growth performance. 3. Effect of dietary gJutamine supplementation on growth performance and plasma biochemical indexes of early-weaned piglets Forty piglets weaned at the 2 Id of age wa randomly assigned to one of two group. The treatment group contained 1.2% glutamine . the diets were calculated to be isonitrogenous and isoenergetic . there were five replicates in each treatment and four pigiets in each replicate. Dietary glutamine supplementation improved growth performance and fred conversion rate of early-weaned piglets, increased markedly plasma albumin content, and decreased plasma globulin content, The actMties of plasma ALP and creatine kinase were significantly high& in trealnient than in control, but the actMties of plasma Y -GT and ALT were lower than in control. The results show tia glutamine supplementation improved growth performance and feed conversion rate of early-weaned piglets, There was important effect on plasma biochemical indexes 4. Effect of dietary glutamine supplementat...