Studies On Biology And Chemical Components Of Seabuckthorn (Hippophae Rhamnoides L.)

Biological characters of root, root nodules, stem, leaves, flower, fruit, seed, seedling and shoot growth length and vigor, fruit seting and yield, chlorophyll contents in leaves, photosynthetic and respiration rate of leaves, fruit development, and change of static state and trends of fruit nutrition components of three seabuckthorn cultivars (China seabuckthorn, Russia seabuckthorn and Mongolia seabuckthorn) from Hippophae rhamnoides L. which were planted at the same ecological condition were studied at contrast manner entirely, it provided scientific foundation for establishing growth measure and processing of seabuckthorn. Structure anatomy of root, stem, leaves, flower and fruit of seabuckthorn were observed by microscope, and pollen and leaf epidermis cuticle piliferous cell were observed by Scaning Electrion Microscope, structure foundation of fight a drought an resest cold from seabuckthorn were founded. Genetic difference of female and male plant from three seabuckthorn were markered at molecule level by RAPD technology, female and male plant of three seabuckthorn can be distinguished at the same time at gene level. Chemical components of seabuckthorn, for example, total flavone, isorhamnetin, quercetin, kaemferin, coumarin, leanolic acid, vitamin C and superoxide dismutase(SOD) were analyzed at contrast manner entirely by PHLC and so on, the results showed that trends change of majority chemical components contents in it appear "M" curve and they have peak near ripeness stage or full-ripe stage. Sort and composition of fatty acids, sterol and vitamin E from juice oil, pericarp oil and seed oil in seabuckthorn were determined by GC-MS, the results indicated that contents of unsaturation fatty acids is higher than saturation fatty acids except China seabuckthorn juice oil in it. Four monocase compounds were picked-up and separated from Mongolia seabuckthorn for the first time. Their structure were identified by mass spectrum(MS) and nuclear magnetic resonance(NMR). The research results provided scientific foundation for growth, breeding, sex identify of female and maleplants, ecology, fastness, processing utilization and traditional Chinese medicine development of seabuckthorn genus plant. The main results are as follows:1 . Biological characters of root, root nodules, stem, leaves, flower, fruit ,seed, seedling and shoot growth length and vigor, fruit set and yield, chlorophyll contents in leaves, photosynthetic and respiration rate of leaves, fruit development, and change of static state and trends of fruit nutrition components of 3 seabuckthorn cultivars (China seabuckthorn, Russia seabuckthorn and Mongolia seabuckthorn) from Hippophae rhamnoides L. which were planted at the same ecological condition were studied at contrast manner entirely. The results showed that growth length and vigor of China seabuckthorn is bigger and stronger than ones of Mongolia seabuckthorn and Russia seabuckthorn. Weight, volumne and yield of Russia seabuckthorn is the biggest and highest in three seabuckthorn cultivars, ones of Mongolia seabuckthorn is bigger and higher than ones of China seabuckthorn. The trends change of weight and volume of three seabuckthorn fruit appear "S" growth curve. Photosynthetic rate of Russia seabuckthorn is higher than one of Mongolia seabuckthorn and China seabuckthorn. The research results provided scientific foundation for establishing growth measure and processing of seabuckthorn.2. Contents of solid matter, sugar, acid, sugar/acid, oil, vitamin E, p-carrotene, amino acids and mineral elements were analyzed and determined. Nutation value of Russia seabuckthorn is higher than one Mongolia seabuckthorn and China seabuckthorn.3. According to the above results, harvest stage was determined with different object. For example, Russia seabuckthorn and Mongolia seabuckthorn fruit harvest stage is sutable during the last ten days of July and China seabuckthorn fruit harvest stage is sutable at the last ten days of August. Leaves made tea harvest stage was choosed...