The Evaluation On Main Characters And The Studies On Sex Makers Of Seabuckthorn

The biology, karyotype, pollen and leaf hair, seabuckthorn oil of China seabuckthorn (H. rhamnoides L. subsp. sinensis Rousi). Russia seabuckthorn (H. rhamnoides L. (subsp. mongolia Rousi cv X subsp. turkestanica Rousi) cv.), Mongolia seabuckthorn (H. rhamnoides L. subsp. mongolia Rousi cv.) cultivated at the same conditions were studied in this paper and used the RAPD to mark their sex gene. The results are as follows:1 The difference of shape and characters of three seabuckthorns seed, Shoot thorn, flower bud and berry are showed in this study. They can be the base for taxonomy. China seabuckthorn has smaller seed, smaller berry with bright color, many Shoot thorns. So it is a very good species for beautification purpose; Russia seabuckthorn and Mongolia seabuckthorn have bigger seed and bigger berry. And it is easy to pick theirs berries. So they are good species for producing.2 It is showed that three germplasms seabuckthorn all have 24 chromosomes and all are diploid, metacentric chromosome. China seabuckthorn and Mongolia seabuckthorn have satellites at 4th chromosome, Russia seabuckthorn at 2rd chromosome. The karyotype all are 2A.3 It is showed that the pollen and leaf hair of three germplasms seabuckthorn used electron microscope scan all are tricolporate and their shapes have little different; The ornamencation of pollen is different. The pollen of china seabuckthorn is smaller with wrinkles at surface; Russia seabuckthorn and Mongolia seabuckthorn are bigger and theirs surface are smooth; China seabuckthorn has most much leaf hair.4 Seed oil and fruit pericarp oil of three germplasms seabuckthorn were extracted by C02 supercritical extraction and berry oil was extracted by centrifugal effct. Component and quantity of seabuckthorn oil was analyses by GC-MS in this study. It is showed that the fruit pericarp oil and seed oil of Russia seabuckthorn and berry oil of Mongolia seabuckthorn are rich in palmy acid and palmy oil acid. The seed oil of Mongolia seabuckthorn is most rich in unsaturated fatty acid. The fruit oil is rich in vitamin E.5 Our study firstly apply RAPD to mark the sex gene of three germplasms seabuckthorn. In this study, it is showed that we only need two primers to distinguish the three germplasms seabuckthorn and their male and female. Russia seabuckthrn and Mongolia seabuckthorn have many same bands. This shows that they are close relative.