| Guided by the theory of animal microecology, a kind of animal microecology preparation that was used in birds and named ChuShengLing was studied and developed in this research. The effects of this probiotic on immunology of chickens were explored systematically and dynamically by using the probiotic product ChuShengLing as experiment material. The results were as follows:1 The developing of probiotic productFrom the rectum interior substance of grown and healthy chicken, five Lactobacillns, twoStreptococcus faecalis lactis, and two Urine Streptococcuses were separated by identifying of growthcharacteristic, morphology observing, and biochemistry test. According cultivated characteristic andanimal safety experiment, two Lactobacillus (LB1, LB2), and one Streptococcus faecalis lactis (SB1)were selected as target microbial of probiotic preparation.The biological characteristics of target microbial were studied. The results showed that LB1,LB2 have some resistant capacity of acid and bile, and can produce acid during progenition.Furthermore they could inhibit the growth of colon bacillus, salmonella, grape cocci andstreptococcus in different degree.Low cost culture medium and production technology of chunshengling were also developed,which offered technology guarantee for factory producing of this product.By determining the bacillus livability under room temperature and stored in dark, we found thatthe stored period of probiotic product ChuShengLing is one year.The effect of probiotic on immunology of chickens and its functionary mechanismIn this experiment, to discover the role of probiotic in immunity and its functionary mechanism, providing scientific guidance for development and application of probiotic some immunology indexes such as numbers of T and B lymphocyte, the content of IgG, IgM, IgA, the T and B lymphocyte proliferative responses in peripheral blood, thymus, bursa of Fabricius, and spleen, the number of Tanae+ cells and IgG antibody-producing cells, inductive activity of IL-2 of T lymphocyte in spleen, the number of Tanae+ cells and IgG antibody-producing cells, antibody-producing cells of IgM and IgA in respiratory tract and alimentary tract local immune tissues, and the content of IgA IgG IgM in tear, trachea fluid, bile, intestinal fluid were determined by using immunohistochemistry, histochemistry staining, indirect enzymelinket immunosorbent assay (ELISA), and MTT method. The results were as follows:After fed with probiotic, the content of IgM, IgA in blood serum of chickens at day 7 was significantly increased as compared with those of the chickens in control group (P < 0.05) . Concurrent significant increase (P < 0.05) of the content of IgG in blood serum at day 10, B lymphocyte proliferation in peripheral blood from days 7 to 18 were also observed, which indicated that humoral immune function in peripheral blood of chickens was significantly intensified by feedingprobiotic.After fed with probiotic, the numbers of T lymphocyte and T lymphocyte proliferation in peripheral blood were significantly increased from days 4 to 10 (P < 0.05) , which indicated that cellular immune function in peripheral blood was also significantly intensified by feeding probiotic.After fed with probiotic, the number of antibody-producing cells for IgM and IgA were significantly increased at day 7 (P < 0.05) , Concurrent increase of IgG antibody-producing cells at day 10 was also observed, which indicated that the growth and humoral immune function of immune organs were increased by feeding probiotic.After fed with probiotic, the numbers of T lymphocyte in thymus and spleen was increased as compared with those of control chicks, which indicated that probiotic could accelerate immune organs growth and the cellular immune function of immune organs were increased.Effects of probiotic on cytokine, T lymphocyte IL-2 inductive activity of thymus and spleen of chickens were studied, lmmunemodulation function of IL-2 in the process of probiotic acting...
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