Geographic Variation And Initial Selection For Wild Provenances Of Polygonum Multiflorum

Polygonum multiflorum is perennial herbaceous vine and it's tuber and vine are used as traditional medicine. Polygonum multiflorum is poplar in many aspects, such as health protection and chemical engineering. The main objective of this thesis is to study difference in genetic, physiology, growth, and chemical compounds among Polygonum multiflorum provenances. The main results are as following: 1. Base on the principle component analysis method and the cluster analysis method, 31 wild provenances of Polygonum multiflorum were divided into three classes by leaf characters. 2. CTAB method was refined to extract high quality DNA, and good reaction system of RAPD marker was built for the species for the first time. 3. 40 pairs primes of high stability and good repeat ability were selected from 300 pairs to amplify. The results showed that wild Polygonum multiflorum provenances had high polymorphic locus percentage (69.5%). The average effective number of alleles in 228 polymorphic loci detected in the test was 1.4556, the average Nei's gene diversity was 0.2781, and the average Shannon's information index was 0.4329. The study showed that wild Polygonum multiflorum provenances had abundant gene diversity. According to the cluster analysis method, 31 wild provenances of Polygonum multiflorum were divided into four classes, generally according with geographic variation of leaf character. The results showed that the relative relationship among different provenances was corresponded with geographical distribution. 4. Vine cutting showed that there were significantly differences in the cutting survival rate among 31 provenances. The tissue culture showed that the proliferation culture prescription used for Polygonum multiflorum bud culture was MS+6-BA1.0mg/L+NAA0.05mg/L and MS+6-BA0.5 mg/L + NAA0.02 mg/L, and the culture medium of rooting was 1/2MS+6-BA1.0mg/L+ NAA0.1 mg/L or 1/2MS+6-BA0.5mg/L+ NAA 0.05mg/L. 5. The study showed that there were significant differences in the growth characters, physiology characters, and quality characters among 10 Polygonum multiflorum provenances. (1) Of all testing materials, provenances in PuDing, HuanJiang and NanJing had fast vine growth; provenances in GaoZhou, TaiShan, and NanJing had best underground tuber growth, and provenances in GaoZhou, HuanJiang and EnShi had best biomass. (2) Net photosynthetic rate of north provenances was a litter higher than that of south provenances. The photochemical efficiency of PSII of south provenances was higher than that of north provenances. There were significant variation on chlorophyll content, solubility proteins content, and nitrate reductaes activity and peroxide enzyme activity among provenances. (3) Among different provenances of Polygonum multiflorum, there were significant variation on content of 6 microelements in the tuber, vine and leaf. (4)　Provenances of in TaiShan, SongXian and EMei had higher the total anthraquinones content than others. However, provenances in GaoZhou and TaiShan had higher the effective economic yield of anthraquinones than others. 6. Relative analysis among characters showed there were significantly negative relativity between 10 characters and the tuber, and 11 different goal character targets were selected out as the important target. 7. By principle analysis and factor analysis, two good provenances were initially selected to be adapted to planting in Jiangsu province.