Identification And Combination Of Different Wheat-Leymus Racemosus Translocation Lines With FHB Resistance

Leymus racemosus Lam., one important species of the genus Leymus in Poaceae Triticeae, was mainly distributed on salina, droughty or half droughty place of North America and middle Asia. Besides the adaptability to poor environments, L. racemosus was also identified with high levels resistance to scab, stripe rust, wheat leaf rust, stem rust, root-rots and BYDV (Barley yellow dwarf virus). The Cytogenetics Institute, Nanjng Agricultural University has engaged in transfer the scab resistance genes from related species or genera since 1980s. A series of wheat-Z,. racemosus addition lines and substitution lines had been produced. DALr.2, DALr.7 and DALr.14 which were added 2,7, 14 chromosomes respectively, showed resistance to FHB. In order to reduce the disadvantages linkage of the alien chromatin, a series of wheat-Z. recemosus translocation lines were induced by ionizing radiation, homoeologous pairing control system, tussue culture and gameteocidal genes etc. But resistance of those translocation lines was not perfect.In this work, 8 different homozygous wheat-Z. racemosus translocation lines were selected by C-banding, FISH, SSR-PCR. 03G1404 was identified to contain two pairs of wheat-Z. recemosus translocation chromesomes. One pair of them consisted mostly of the chromesome 4B and short arm of Lr.2 (T4BS ? 4BL-Lr.2S). The other pair translocation chromosomes consisted partly 4BL, and Z. racemosus chromosome segment with the centromere which was still not conformed. 031428 was identified to contain a pair of wheat-Z. recemosus translocation chromosomes which consisted of most Lr.7 of L. racemosus and a wheat chromosome segment (TLr.7L ? 7S-W). Translocation chromosomes in 03G1656, 03G1522 and 03G1548 were identified to be centric fusion translocation chromosomes (TLr.7S ? W) which consisted the short arm of Lr.7 and...