Molecular And Cytogenetic Identification Of Triticum Aestivum-Leymus Racemosus Alien Translocation Lines

Wheat scab(Fusarium head blight,FHB) is one of the most disastrous diseases in the world and increases with the development of warmhouse effect.Transfer of scab resistance from wild relatives to common wheat is a useful method.Leymus racemosus has high resistance to wheat scab.So far,a series of wheat-L.racemosus addition and substitution lines have been developed.By use of gametocidal effect of Aegilops cylindric chromosome 3C,many wheat-L.racemosus translocation lines were developed in cytogenetics institute, Nanjing Agricultural University(CINAU).But most translocations' identities were not confirmed because of their complex or non-characteristic chromosome bands.In this study, by use of the translocation lines involving Lr.7 which have been identified roughly as the basic material,three wheat-L.racemosus translocation lines were identified accurately through using fluorescence in situ hybridization(FISH) with genome-specific BACs, 676D4 and 9M13,plasmid clone,pAs1 and pSc119.2,45SrDNA,as probes,test-crossing with ditelosomics,and satellite sequence repeat(SSR) analyses,in combination with chromosome banding and genomic in situ hybridization(GISH).The translocation lines are WL06(T3AS·Lr7S),WL16(T6BS·Lr7S) and WL33(T5AS·Lr7L).