Study By Molecular Cytogenetics Technique On The New Wheat Lines Which Had Been Introduced The Desirable Genes Of Leymus Multicaulis

The number of chromosomes of the hybrid offspring between the wheat and leymits multicaulis was detected in this study. From the 12 kinds 15 Disomic alien addition lines which had been detected in 1992, we had found 11 kinds 12 lines which were 2n=22", and we had only found the 2n=21"+1' individual in LineS. From other offspring of hybrid between the wheat and leymits multicaulis, we had obtained 9 lines which were 2n=22". 10 lines which were 2n=44. 2 lines which were 2n=21"+1' and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group. By C-banding detection and karyotype analysis, we had obtained the standard C-banding and karyotype of three kinds of forage, that is. Puna Chicory, leymus multicaulis and Psathyrostachysjuncea.For the materials used in this article were some old seeds which had been stored for 3-7 years, we found a lot of abnormal mitotic and meiotic cells, such as chromosome bridge. chromosome laggard, micronucleus, multipolar division and so on. Moreover, we also found the wheat's multi-anther, double germinate-hole's microspore and collected the photos of every period of meiosis. mitosis. To make the best of using in the teaching, we had edited the "cytogenetics teaching courseware". This courseware include the photos and techniques of C-banding, FISH, SSR and the photos which had introduced in the above paragraph, and some flash software just for teaching use.