EAG Responses Of Asian Longhorn Beetle Anoplophora Glabripennis(Coleoptera:Cerambycidae)to Volatiles Of Hickory

Asian longhorn beetle Anoplophora glabripennis(Coleoptera:Cerambycidae) is arguable the most destructive insect of Carya cathayensis in Lin’an city.The volatiles of Carya cathayensis play important roles in the behavior of Anoplophora glabripennis, such as host selection, attack, oviposition and reproduction.Antenna is the major organ of the insects to detect host volatiles and electroantennogram(EAG) techniques have been widely used in chemical ecology to identify olfactory active compounds and characterize antennal receptor neurons.Therefore, to better understand the mechanism of the dispersal and aggressive behavior of Anoplophora glabripennis.So that a detailed research on the EAG reponse of Anoplophora glabripennis to the volatiles of Carya cathayensis is required. In order to analyze different emission patterns of hickory plants in different condition, adopting dynamic head-space and GC-MS, volatiles of living hickory plants in nature were successfully collected and analyzed.20 volatile components were identified.The results of the experiments showed that volatiles displayed significantly different emission patterns between mechanical wounding plants and insect herbivore plants.Healthy plants release 10 kinds of volatiles, mechanical wounding plants release 10 kinds of volatiles.There are 5 different kinds of volatiles.Longicorn beetles damaged plants release 17 kinds of volatiles.Comparing with mechanically damaged hickory plants, there are 10 different kinds of volatiles. Electroantennography(EAG) instrumentation was used to examine the EAG response of Anoplophora glabripennis adults to nine kinds of Carya cathayensis(hickory) volatiles, including: α-pinene, β-pinene, ocimene, 1,4-dichlorobenzene, 1,2-xylene, camphene, limonene, camphorand and so on, at five concentrations. Significant differences(P < 0.05) were present for the feeling strength of the antennae in relation to volatiles from female and male adults. For female adults β-pinene elicited the highest EAG response(relative EAG values is about 17.4), for male adults EAG responses to limonene and ocimene were appresent(relative EAG values is about 13.4 and 7.32). In addition, EAG showed a response to different concentrations of volatile matter for tentacles, significantly different among different samples(P≤0.05) by LSD method. This study will provide a theoretical foundation for research and development of attractants and repellents of Anoplophora glabripennis.