| Is this paper, Kunming mice were used to study the effect of flavonoids separated and purified from Allium mongolium Regel on immity and antioxidant function in body. The experiment was divided into three parts:The first part of the experiment determined the best extracting and purifying conditions of the flavonoids from Allium mongolium Regel,75% ethanol c used to extract flavonoids in Allium mongolium Regel,extraction temperature was 70℃,extraction time was 2 hours/2 times. Polyamide column chromatography was used to separate and purify flavonoids,using 30%,50%,70% and 95%ethanol washed and then the structure of flavonoids from Allium Mongolium Regel was identified by liquid chromatography -ESI-tandem mass spectrometry technology (LC-ESI-MS-MS).The results showd that:eight kinds of flavonoids from Allium mongolium Regel were separated by 30% ethanol They are,kaempferol-4′-O-3,7-O-O-2glucosidase(m/z609), quercetin-7- O- 3-O -rutaceaeglucoside(m/z609),kaempferol-4'-O-7-O-glucose-3-O-2glucosidase(m/z771),7-O-5,4′-dimethoxy-3'-hydroxyflavonol(m/z329),3′,4′-epoxy-7-O-5-methoxy-flavonol(m/z327),quercetin-7-O-3-O-2glucosidase(m/z625),quercetin-7-O-3-O-glucoside(m/z463) and kaempferol-7-O-3-O-glucoside(m/z447) respectively; seven kinds of flavonoids from Allium mongolium Regel were separated by 50% ethanol they are, Luteolin -5'-O-glucose-4-hydroxy phenylpropionate(m/z593),quercetin-7-O-3-O-rutaceae glucosi -de(m/z609),kaempferol-4'-O-3,7-O-O-2glucosidase(m/z609),7-O-5,4′-dimethoxy-3'-hydroxyflavonol(m/z329),3′,4′-epoxy-7-O-5-methoxy-flavonol(m/z327),quercetin-7-O-3-O-glucoside(m/z463) and kaempferol-7-O-3-O-glucoside(m/z447) respectively; three kinds of flavonoids from Allium mongolium Regel were separated by 70% ethanol they are,7-O-5,4′-dimethoxy-3'-hydroxy flavonol(m/z329),3',4',3,4- 4hydroxy-6-O–flavonoid (m/z273) and 3′,4′- epoxy -7- O- 5- methoxy-flavonol(m/z327) respectively; two kinds of flavonoids from Allium mongolium Regel were separated by 95% ethanol they are,3′,4′-epoxy-7-O-5- methoxy-flavonol(m/z327) and 7-O-5, 4′-dimethoxy -3'- hydroxyl flavonol(m/z329) respectively.In the second part of the experiment,480 (weight 20±2g) pure-blooded Kongming mice, which were divided randomly into four groups and each group 120 (half female and half male), were raised in different cages which were named control group, 75.36mg/kg·BW·d Allium mongolium Regel supplementation group,75.36mg/kg·BW·d flavonoid extract supplementation group,75.36mg/kg·BW·d flavonoids purification supplementation group. Preparatory experimental period lasted for 5 days and the formal experimental period lasted for 14 days. After two slaughters being done during the formal experimental period, two slaughter experiment were conducted,the blood was collected and serum was isolated ,and live thymus and spleen was dissected and weighed.The result show that, the content of T-SOD and GSH-Px in serum to Allium mongolium Regel group and flavonoids purification group was higher than that of control group and flavonoid extract group(P<0.05). The content of CAT in serum of flavonoids purification group and flavonoid extract group was higher than that of control group(P<0.05).The content of MDA in serum of experiment groups was lower than that of control group, but the difference was not significant(P>0.05).The content of T-AOC in serum of experiment groups was higher than that of control group(P<0.05).The content of T-SOD in liver of experiment groups was higher than that of control group(P<0.05), but the difference was not significant in experiment groups(P>0.05).The content of GSH-Px in liver of flavonoids purification group was higher than that of other groups(P<0.05).As compared to the control group, The CAT content in liver of flavonoid extract supplementation group was significant lower(P<0.05).The content of MDA in liver of Allium mongolium Regel group and flavonoids purification group were lower than that of control group and flavonoid extract group(P<0.05).The content of T-AOC in liver of flavonoids purification group was higher than that of other groups, but the difference didn′t reach statistical significant(P>0.05).From the results we conduded that flavonoid of Allium mongolium Regel can enhance the content and activity of antioxidant enzymes in serum and liver and reduce the content of lipid peroxidation in vivo, thereby enhance the body's antioxidant capacity.The design of the third part of experimental was the same as the second part. The results showed, the spleen index of experiment groups were higher than that of control group, but the difference was not significant (P>0.05).The thymus index of flavonoids purification group was higher than that of other groups(P<0.05).The content of ACP in serum of flavonoids purification group was higher than that of other groups(P<0.05); The carbon clearance index of experiment groups was higher than that of control group, but the difference was not significant (P>0.05).The phagocytic index and lysozyme content in serum of flavonoids purification group were higher than other groups(P< 0.05),the content of lgG in serum to flavonoids purification group was higher than that of control group(P<0.05).The number of CD19~+ of flavonoids purification group was higher than that of other groups, but the difference was not significant (P>0.05).The number of CD4~+ of flavonoids purification group was higher than that of control group and Allium mongolium Regel group(P<0.05).The number of CD8~+ of control group was higher than that of other groups(P>0.05).The content of NO in serum of experiment groups was higher than that of control group, the content of NO in serum of flavonoid extract group was higher than that of control group(P<0.05), flavonoids purification group(P<0.05) and Allium mongolium Regel group(P>0.05).The results showed that flavonoids purification supplementation group can enhance immune organs index, the content of ACP and lysozyme in blood and liver, thereby enhance the capability of phagocytes to digest and degradate of foreign body. Meanwhile, carbon dissection index(κ) and engulfed coefficient(α) of mice in flavonoids purification group was significantly increased,so flavonoid of Allium mongolium Regel played an important role in enhancing the body non-specific immune function.Flavonoid of Allium mongolium Regel can improve the content of IgG and the percentage of CD19~+ (B cell) in the peripheral blood of mice. The results indicated that flavonoid of Allium mongolium Regel stronghten the immune function of body fluid; Through increasing the number of antibody in the body formatting cell and enhancing the function antibody formation of cell.Through increasing the cell numbers of CD4~+ and CD8~+ in blood, flavonoid of Allium mongolium Regel enhanced humoral immune function. Flavonoid of Allium mongolium Regel activated macrophages by creasing the content of NO,which was biological information transmission and play the non-specific sterilization and killing tumor function of macrophages and carry through complex immunization activities with participation with T lymphocytes and B lymphocyte.
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