The Regulation Of The Procreation Incretion Of The Embryo Transfer On The Sheep And The Recipient's Effects

The research chose German Mutton Merino 15♀, Borderdale 15♀, and Poll Dorset 18♀with theage of 14 months as the donor of embryo's sheep, and the choice of Small-tail Han sheep 124♀(24months, 2births) as another receptor group. The donors have undergone the estrus synchronization and multiple ovulation by the treatment of CIDR+PMSG+FSH; the receptors have experienced the corresponding estrus synchronization by the CIDR+PMSG. It compared with the dynamic change of P₄, E₂, FSH and LH in the peripheral blood of German Mutton Merino,Borderdale, Poll Dorset and Small-tail Han sheep, and analysised the effects of estrus synchronization, superovulation and embryo transfer of German Mutton Merino,Borderdale,Poll Dorset and Small-tail Han sheep. And meanwhile it reaserched postpartum (0-96h) receptors' transferring ability of IgG in the colostrum and the cumulative growth effect produced by receptors to their offspring(0-12month).At the 13d, the donor and the receptors removed CIDR; at the 14d and the 15d, there are 41 and 4 donors respectively in Synchronization of Estrus, while at the 14d, 15d and 16d, there are 7, 81 and 9 receptors respectively in Synchronization of Estrus. As for the luteal number and ovum number ,The mean number in German Mutton Merino,Borderdale and Poll Dorset is 5.50, 4.07, 3.44 respectively, German Mutton Merino is significantly higher than that of Poll Dorset (P<0.05), the mean of available embryos is 3.86, 2.27 and 2.69 respectively; and the pregnancy rate of ET in those is 55.26%, 54.17%, 42.31%, German Mutton Merino is significantly higher that of Poll Dorset (P<0.05) .In the first day of natural estrous cycle, the contents of FSH (5.19mIU/ml) in the serum of Small-tail Han sheep of receptors is significantly higher than Borderdale (P<0.05) , as well as the contents of LH (3.66mIU/ml) is significantly higher than Poll Dorset (1.32mIU/ml) (P<0.05). In the 8d of natural estrous cycle, the contents of LH (3.45mIU/ml) in the serum of Small-tail Han sheep of receptors is significantly higher than Poll Dorset (1.18mIU/ml) (P<0.05) ; In the 16-17d of estrous cycle, the contents of P₄ (0.93ng/ml) in the serum of Small-tail Han sheep is significantly higher than Poll Dorset (0.27ng/ml) (P<0.05), as well as the contents of FSH (7.86mIU/ml) is significantly higher than Poll Dorset (3.76mIU/ml) (P<0.05). The peak of P₄ of German Mutton Merino, Borderdale, Poll Dorset and Small-tail Han sheep in the dynamical change curve is respectively 10.12ng/ml, 9.14ng/ml, 8.78ng/ml, 12.17ng/ml, the peak of E₂ in the dynamical change curve is respectively 8.34pg/ml, 7.43pg/ml, 6.44pg/ml, 9.34pg/ml, and the peak of FSH in the dynamical change curve is respectively 5.11mIU/ml, 3.76mIU/ml, 4.19mIU/ml, 7.86mIU/ml, as well as the peak of LH in those is respectively 5.87mIU/ml, 3.66mIU/ml, 3.82mIU/ml, 6.22mIU/ml in the estrous cycle; The whole change trend of P₄,E₂,FSH,LH is that, Small-tail Han sheep is higher than German Mutton Merino, Borderdale, Poll Dorset, German Mutton Merino and Small-tail Han sheep are similar in quantitative characteristics and quantitative characteristics, the next is German Mutton Merino. Exogenous reproductive hormone regulation changed completely for reproductive hormone P₄, E₂, FSH, LH secretion rhythm in donor and receptor, the donor and receptor for estrus synchronization experience than less experienced natural estrus2-3d, reproductive hormones P₄, E₂, FSH, LH secretion rhythm and volume relatively similar in serum of treatment period of German Mutton Merino, Borderdale, Poll Dorset, larger variation is Small-tail Han sheep; The first day in treatment period, receptor Small-tail Han sheep P₄ concentration in serum (5.66ng/ml) was significantly higher than that of German Mutton Merino (2.40ng/ml) (P<0.05), FSH (4.09mIU/ml) was significantly higher than that of Borderdale (1.04mIU/ml) (P<0.05), on 8d, LH (3.17mIU/ml) in Small-tail Han sheep serum were significantly higher than those Borderdale (0.94mIU/mL) (P<0.05) and Dorset (0.96mIU/mL) (P<0.05); P₄ concentration in serum of German Mutton Merino, Borderdale, Poll Dorset, Small-tail Han sheep in the first 13d will reach its peak, were 8.77ng/ml, 7.97ng/ml, 8.77ng/ml, 14.14ng/ml;in the 12d of treatment, E₂ curve of the three foreign varieties began increasing rapidly, and Small-tail Han sheep in the 13d E₂ curve still in the lowest point and less than the levels in the peripheral blood of three foreign varieties in the 12d, natural estrus cycle German Mutton Merino, Borderdale, Dorset, Small-tail Han sheep E₂ did not change the characteristics of rhythm, E₂ reached its peak in estrus, German Mutton Merino, Borderdale, Poll Dorset, Small-tail sheep were 6.67 pg/ml, 5.97pg/ml, 5.92pg/ml, 7.92pg/ml; German Mutton Merino, Borderdale, Poll Dorset FSH curve reached its peak in the 13d, were 5.89mIU/ml, 5.07mIU/ml, 4.98 mIU/ml, and Small-tail Han sheep in the 14-15d will reach its peak, its content was 7.37 mIU/ml, the natural estrus cycle FSH such characteristics have not changed; The peak of LH curves of German Mutton Merino, Borderdale, Poll Dorset, the Small-tail Han sheep, were 6.87mIU/ml, 6.02mIU/ml, 5.27mIU/ml, 6.89mIU/ml; Compared with the peak of P₄, E₂, FSH, LH curve of Natural reproduction estrous cycle, P₄ and LH of Small-tail Han sheep are at increased state, E₂ and FSH at a lower state; P₄, E₂ of three varieties at a lower state, FSH, LH are at increased state.After the embryo of German Mutton Merino was transferred to receptor small-tail Han sheep Oh, the IgG content of whey (89.43mg/ml) was significantly higher than that of natural reproduction German Mutton Merino (54.32mg/ml) and Small-tail Han sheep (48.66mg/ml) and the content in serum of itself (66.22mg/ml) (P<0.05), 12h IgG content in whey (76.11mg/ml) was significantly higher than IgG content (P<0.05)in serum of natural reproduction German Mutton Merino (50.26mg/ml) and Small-tail Han sheep (46.77mg/ml). IgG content in whey of Small-tail Han sheep receptor in 12h, 24h, 36h significantly higher than that of natural reproduction German Mutton Merino and small-tail Han sheep (P<0.05), 48-84h, IgG content in whey of small-tail Han sheep receptor still higher than that of natural reproduction German Mutton Merino and Small-tail Han sheep. There were not IgG was detected in serum at Oh before lamb eat colostrum, after natural reproduction German Mutton Merino lamb, Small-tail Han sheep lamb, and German Mutton Merino lamb from embryo transfer have eat colostrums 24h, the IgG content in serum reached maximum, respectively, 60.11mg/ml, 61.22mg/ml, 66.15mg/ml, 24h-96h continuous decline, 60h-96h, the IgG content in serum of German Mutton Merino lamb by embryo transfer higher than the rate of decline in natural reproduction German Mutton Merino lamb and small-tail Han sheep lamb, at 96h, IgG content in serum of the natural reproduction German Mutton Merino lamb, Small-tail Han sheep lamb and German Mutton Merino lamb by embryo transfer were 26.69 mg/ml, 24.67mg/ml, 15.23mg/ml.The cumulative growth volume of natural reproductional German mutton merino, Borderdail and Poll Dorset all at the age of 12-month-old reached 72.17kg, 75.34kg, 73.54kg; and the reached 63.17kg, 61.44kg, 64.34kg respectively, and in different month cumulative growth showed significantdifference with natural reproductional (P<0.05). Using Logistic model (logistic model)Y =K/1+e^a-bt,we could study the character of cumulative growth of natural reproductional German mutton merino, Borderdail, Poll Dorset and transplanted German mutton merino, Borderdail and Poll Dorset, and Small-tail Han sheep. The duration cumulative period of increasing growth and rate of Natural reproductional German mutton merino, Borderdail and Poll Dorset were 2.15 months respectively, and 4.63kg/month-old, 3.32-month-old and 4.07kg/month and 1.91months and 4.79kg/month respectively, and continuing a long fast period duration and rate were7.57months and 4.63kg/month-old, 8.44-month-old and 4.07kg/month and 7.25months and 4.79kg/month, the durative increasing period with a tardo situation and rate were 9.43months and 0.27kg/month, 10.50months and 0.27kg/month and 9.03months and 0.28kg/month respectively. And the ransplanted were 4.31 kg/month and 3.78months and 3.14kg/month and 2.97months and 4.18kg/month respectively, and the continuing a long-term fast durative time and rate were 9.15months and 4.31 kg/month, 10.13 months and 3.14kg/month and 7.83 months and 4.18kg/month, and the durative increasing period with a tardo situation and rate were 0.26kg/month and 11.39 months, 12.61months and 0.19kg/month and 9.87months and 0.25kg/month respectively.