Ecological Adaptability And High Efficiency Propagation System Of Lonicera Caerulea

Blue honeysuckle(Lonicera caerulea Linn.) has a high consumption,health care,medicinal and ornamental value.Russia,Japan,the United States and other countries attach great importance to this new green fruit resource,which has broadened the prospects for further development.China is rich in wild blue honeysuckle,however,no researches have been conducted on its communities or population ecology.And researches into physiological and ecological basis in cultivation,breeding,and quantitative cultivation technology have just begun.Based on the above understanding,this study was conducted from 2004 to 2009,with the wild blue honeysuckle distribution area on the northern slope of Changbai Mountain as the main research sites,and Jilin Province Jiaohe Experimentation Administration Bureau and Tree Garden of Beihua University as the supporting research sites.At individual,population and community levels,from micro to macro,systematic studies were carried out on high efficiency propagation system of blue honeysuckle and its ecological adaptability by using phenology,ecology,botany,silviculture and other multi-disciplinary principles and methods in sample field investigation and point observations.The studies were aimed at providing a scientific basis for the practice and decision-making concerning blue honeysuckle domestication,genetic diversity conservation,sustainable use of plant resources and the enhancement of population productivity.In this research,based on a large number of field investigations on elevation gradients of the northern slope of Changbai Mountain,in-depth studies were carded out on blue honeysuckle species diversity,interspecific association,and the impact of disturbance on community diversity,which led to population life table,population survival curve,in-depth study of blue honeysuckle population structure and dynamics,and analysis of the spatial distribution pattern of the population.The total of blue honeysuckle community on the northern slope of Changbai Mountain was 187,of which there were 42 species of trees,37 species of shrubs,and 108 species of herbs.Community life-form was complex,including 77 phanerophytes(41.2%),6 chamaephytes(3.2%),54 hemicryptophytes(28.9%),47 geopnytes(25.1%),and 3 therophytes(1.6%).Blue honeysuckle was the dominant species in the shrub layer in its community,sub-dominant species were blueberries,big-leaf rose,kraft azalea and single-flower honeysuckle and so on.Its coverage reached more than 90%at about 1200 m above sea level,shrub height ranging from 0.2 to 2 m.Through comprehensive study on blue honeysuckle morphological variation,leaf morphology indicators,ecological indicators,anatomical structure,thermal value and the main substances at elevation gradients,together with correlation analysis and multivariate statistical analysis of leaf characteristics of indicators and environmental meteorological factors,soil factors and stand factors,the real cause for indicator changes in leaf characteristics was analyzed,and in-depth study on ecological adaptation of blue honeysuckle at elevation gradients was made.With the changes of elevation, corresponding changes occurred in every environmental factors,which to some degree affected blue honeysuckle morphological structure and ecological characteristics.At altitude gradients changes in various forms occurred in different organs of blue honeysuckle,which was the result of the impact of environmental factors as well as the adaptation to environmental changes.Blue honeysuckle had adjusted its structure,its morphological,ecological indicators,caloric value and the main substance content to the high mountain environments.Biological zero degree of blue honeysuckle was 0.3℃.There was a high level of GA at the beginning of blue honeysuckle inflorescence,which then progressively declined.High levels of GA were not conducive to the formation of flowers;low ratio of ABA/IAA is conducive to the formation of flowers.In the latter part of fruit growth,low levels of IAA,GA,ZR,high levels of ABA resulted in blue honeysuckle fruit drop.A comprehensive study on blue honeysuckle in vitro culture techniques through the shoot and callus found that 6-BA,IBA and KT,used in conjunction,could increase the number of rosette buds of blue honeysuckle subculture.Decrease in the macroelements,microelements,iron salts and organic matter concentration in MS medium was conducive to blue honeysuckle rooting culture.6-BA and IBA, used in conjunction,was conducive to bud induction,while the NAA used in conjunction with the 6-BA was conducive to callus induction.The results showed that appropriate program for the culture of early generation was MS+6-BA 1.0 mg/L+IBA 0.2 mg/L,appropriate program for subculture was MS+6-BA 0.5 mg/L+IBA 0.3 mg/L+KT 1.5 mg/L,the best medium for callus was MS+6-BA0.10 mg/L+ NAA1.0 mg/L,the best medium for callus differentiation was MS+6-BA3.0mg/L+NAA0.01 mg/L+ KT1.5 mg/L.,appropriate program for rooting culture was 1/4MS+IBA 1.5 mg/L,survival rate of rooting plantlets,after domestication and transplantation,was up to 97.8%.Systematic studies on blue honeysuckle hardwood cuttings technology and cuttings rooting mechanism were made,therefore, provided an effective way for the rapid propagation of this tree species.The best environment temperature for blue honeysuckle seed germination was 21℃,soaking time 4 d.Gibberellin had no obvious effects on the germination rate,while it had a very significant effect on seed germinating potential(P＜0.01).Desirable gibberellin content for improving blue honeysuckle germination was 100-500 mg/L.Stratification had a significant effect on both germination rate and germinating potential.Temperature stratification being 10 d before planting in spring,the seed germinating potential reached the highest(44.3%),and the germination rate was relatively high (84.0%).