Theoretical And Experimental Research On The Pathological And Physiological Base Of Spleen Failing To Control Blood Syndrome In Dysfunctional Uterine Bleeding

The research systemically investigated the pathological and physiological base of spleen failing to control blood syndrome in dysfunctional uterine bleeding through the theoretical and animal experimental study.In the theoretical study, spleen failing to control blood syndrome, DUB, and metrorrhagia were mainly discussed. First, by literature search and statistical analysis, we concluded that the syndrome of spleen failing to control blood was mostly seen in DUB. In ancient times, the Chinese traditional medicine treatment to the spleen failing to control blood gave priority to supplementing to cure interior disease, in common using rnongolian milkvetch root, ginseng, liquorice, largehead atractylodes rhizome, et al tonifying spleen for nourishing qi drugs. At the present day, besides mongolian milkvetch root, largehead atractylodes rhizome, tangshen, Chinese angelica, ass-hide asafetida, et al tonifying spleen for nourishing qi and hemostatic drugs, sanchi, agrimony, garden burnet root, India madder root, et al hemostatic drugs were also used to treat hemorrhage. In conclusion, the herbal medicine to treat the spleen failing to control blood syndrome involved nourishing qi to invigorate spleen and tonifying blood drugs and hemostatic drugs. Its composition was similar to decoction for invigorating the spleen and decoction for strengthening middle energizer for nourishing qi. They are frequently used prescription to treat the spleen failing to control blood.Secondly, the diagnostic criterion, pathogenesis, syndrome differentiation, and Chinese tradition medicine treatment to DUB were discussed in detail. The pathogenesis of DUB was generalized as three aspects. ㏕he influence of neurotransmitter and hormone in pallium, hippocampi, hypothalamus et al; ㏕he changes of estrogen on hypothalamus-pituitary-ovary axis; (3)The changes of endometrium, including cell apoptosis of endometrium, incretion agents, and local blood vessel and the components of blood. A lot of physicians' clinic experience approved the syndrome of spleen failing to control blood was common and important to DUB. Its treatment theorem was nourishing qi to invigorate spleen and hemostasis. The literature investigation indicated that mongolian milkvetch root, largehead atractylodes rhizome, tangshen, et al nourishing qi to invigorate spleen drugs and ass-hide asafetida, sanchi, garden burnet root, et al hemostatic drugs were more applied.Then the pathogeny and pathology, syndrome differentiation and typing, symptom andtherapeutical principle and technique of metrorrhagia were systemically expatiated. There were lots of etiological factors to cause metronhagia. Its pathogenesis included splenic asthenia, renal deficiency, cardiopenia, hepatic depression et al viscera injury; hemopyrexia, hemostagnant, asthenia of qi et al qi and blood disorder. It could be induced as deficiency, febris and stasis. Syndrome differentiation to metronhagia could be typed sthenia pyrosyndrome, asthenia pyrosyndrome, asthenia of renal yin syndrome, ashtenia of renal yang syndrome, splenasthenia syndrome, and hemostagnant syndrome. Triple therapy for metrorrhagia was commonly used to treat it.The spleen of Chinese medical science is the foundation of acquired disposition. It can generate qi and blood, control blood. It also is the pivot of qiactivity hoisting. It relates closely to the occurrence of hemorrhagic disease and woman physiology, psychology, and menstruation. So the spleen acts an important role in the occurrence of DUB and metrorrhagia. The spleen failing to control blood is their common syndrome.In the experimental research, the methods of swimming, diarrhea, and bleeding factor were applied to build the spleen failing to control blood animal model. Radioimmunoassay, flow cytometry and hybridization in situ with image analysis were used to detect estradiol, progesterone, 6-keto-prostaglandinFi ? thromboxane 62, P -endorphin, apoptosis and cell cycle of endometrium, POMC mRNA gene expression of brain tissue and so on. At the same time, the pr...