| Objective: To probe into the basic research on extraction of available compound substances by semi-bionic extraction (SBE) and take example for Fuzhuang Capsule.Methods: Using the even designment to optimize the best extraction conditions of the semi-bionic extraction for Fuzhuang Capsule, withicariine, /?-sitosterol, betarine, panaxadiol, panaxitriol, total saccharine and dried extract weight as the indexes, in addition, using total integral calculus area of absolute alcohol solution by HPLC to optimize the extraction methods of combined ingredients of Fuzhuang Capsule. HPLC has been used for the finger print chromatograms of different samples. Principal components analysis, cluster analysis and stepwise recognition analysis have been used for the pattern recognition. Optimizing the alcohol precipitation concentration using proportional method. According to the former conditions, the four kinds of extract methods, including the semi-bionic extraction (SBE),the water extraction (WE), the semi-bionic extraction by precipitation with alcohol (SBAE) and the water extraction by precipitation with alcohol (WAE), were compared in the contents of the index ingredients, in the pharmacological activity and in the fingerprinting of the rabbit's blood serum. Based on the former research, Fuzhuang Capsule was prepared.The quality control standards were established and the stability of the preparation was studied.Results: The technological conditions for SBE: the pH values of the water for the 1st, 2nd, and 3rd decoctions are in order of 4.0, 7.5, and 8.7, and the extraction times are 150min, 75min, and 75min respectively. Mixing herb Epimedii(A), herb Cistanchis(B), fructus Lycii(C) and radix Ginseng(D) together to extract is the best combination.The results of technological conditions and the extraction methods of combined ingredients of Fuzhuang Capsule by SBE are tested and found to be correct byfingerprint chromatograms and pattern recognition. The sequence of total index Y value of five kinds of alcohol precipitation concentration is:Y61.25%>Y55%>Y67.5%>Y30%.>Y80%. Combined with the production, the best alcohol precipitation concentration is 60%.The sequence of total index Y value of the four extraction technology is:YsBE>YwE> YSBAE>YWAE- Using HPLC for completely comparing the ingredients of SBE and WE, the result shows that the number and the place of the peaks in SBE and WE basically are the same, the integral calculus of SBE is all above WE besides one.It indicates that there has been no change in the nature of SBE and We, but the content in SBE is obviously high than in WE, SBE is the best extraction method in preparation of Fuzhuang Capsule.The four kinds of extractions especially SBE can raise the concentrations of testosterone in blood and lowered levels of both bata-EP in the hypothalamus and frontal contex in senile rats. SBE method can obviously promote the content near to young animal's level.It is indicated that SBE can effectively improve the immunity function and make body stronger than WE. The HPLC fingerprinting and the HPCE fingerprinting of the rabbit blood serum show that the SBE decoction's metabolic rate is more rapid than the WE decoction in vivo, SBE is easier to be absorbed than WE in the human body.Fuzhuang Capsule was prepared. Using TLC and HPCE to identify the medicinal materials.The quality control standards were established and the content range of the index ingredients are: icariine above 0.85mg ?g"1, ^-sitosterol above 12.8 ng ?g"1, betarine above 8.9mg ?g"1, panaxadiol above 8.9 P g ?g"1, panaxitriol above 3.2 u g ?g"1. The study of the preparation's stability shows that the expiration date of the capsule is 2 year.Conclusion: Prepared in accordance with semi-bionic extraction patterns, Fuzhuang Capsule has high contents of chemical compositions , good curative effects, stability and being controlled quality and taken convenience. The established semi-bionic extraction patterns provide a new way of thinking and method for compound substances and development for...
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