Studies On Huanglian Jiedu Decoction To Probe Into Semi-bionic Extraction Mode

Object Take Huanglian Jiedu Decoction as an example to probe into the basic mode on extraction of available substances in Chinese compound medicine by semibionic extraction.Methods With Determining,standardizing,weighting and evaluating the contents of berberine,baicalin,geniposide,total alkaloid,total flavonoid,total integral calculus area of HPLC and extract of l000Da, researching the Huanglian Jiedu Decoction's Identification of the sources, optimizing SBE technical conditions, the best extracting mode and the best concentration of alcohol extract;Then, comparing 3 kinds Extract of 1000Da of SBE,AE,WE, by study the index component; finally comprehensively evaluating the best technology of Huanglian Jiedu Decoction.Result The optimized SBE extraction conditions are the following:pH values of the water for three extraction is 6.0,7.5,8.5, and extraction time is 2.0h,1.0h,1.Oh in turn; The optimal combination is Rhizoma coptidis and Fructus Gardeniae, Radix Scutellariae and Cortex Phellodendri Amurensis to be decocted respectively before the decoction is combined finally; the best concentration of alcohol extract is 60%;The best comprehen-sive evaluation of 3 kinds Extract of 1000Da is SBE; The strongest effect of 3 kinds of serums and extracts of l000Da is SBE. Conclusion Comparison of the active ingredients, the best extraction of Huanglian Jiedu Decoction is SBE. The test is the typical of Semi-bionic Extraction Mode on compound that doesn't contain volatile ingredients...