| Endoscopic sinus surgery, as a therapy of nasal cavity and sinus diseases, had extensively developed in home and abroad at present. Although most patients with the original nasal cavity and sinus diseases were cured after endoscopic sinus surgery, long peri-operation was still existing because the nasal massive effusion and crusts must be cleaned again and again, moreover, a few patients would be observed some complications such as nasal cavity adhension, or occlusion and secondary sinusitis, and so on. At present, these complications were thought by many scholars to be related to maloperation, lesion or anatomy variation of middle nasal turbinate, infections and the degree of lesion and so on (1, 2), while the injuries of nasal mucosa induced by light and heat in transnasal endoscopic sinus surgery were neglected, thus we observed these injuries through animal experiments.Through this experimental study, we believed that these complications were also concerned with light and heat in transnasal endoscopic sinus surgery, were induced by a kind of complex injury. We observed that the cilia of group A(normal control group) were well-distributed and the cilia's thickness were idendical, their height and lenth were not distinctly different, and the typical microvillares structures that "9+2" could be seen. Meanwhile, massive slender mitochondria that cristae were well-arranged and the matrix were obviously could be seen. Mucus secreted particles in goblet cells were intensive. The cilia of group B(continuous irradiation for half an hour) distributed poorly. Their adherense, exfoliation and budding growth could be observed. The distributions of the cilia in group C(continuous irradiation for an hour) were sparse and the degrees of their thickness were different, the mitochondria were condensation, the nuclei of epithelium cells were edge-located, the distributions of the chromatin were mass, concentrated by the fringe, the nuclei were close to the cell surface and the exfoliations of goblet cells were visible. In group D(10 minutes irradiation for intermission 6 times), the spacing of the epithelium cells increased, part of cilia's adherense, exfoliation and budding growth were visible.The mitochondria were highly bloated, accompany with disappeaance of the cristae and vacuolation of matrix. In group E(20 minutes irradiation for intermission 3 times), the cilia were maldistributed, had not directivity, part of them were adherent and deciduous.The mitochondria were highly bloated, accompany with disappeaance of the cristae and vacuolation of matrix.Because of the irradiation of brevity and high intensity in this experimental, the changes of mucosal chronic inflammation such as proliferations of many goblet cells and fibro-vessels, decreased ciliated cells and infiltrated inflammatory cells were not observed in above groups, so the injury of nasal mucosa induced by light and heat in this experiment was different with nasal and nasal sinus chronic inflammation. At the same time, we observed budding growth of cilia which mechanism wasn't clear, so more experimental studies were performed. The studies about these complications mainly focused on machinery and biotical causes before, while the studies about the repair and regeneration after injury of nasal mucosa induced by light and heat were needed to clarify.To date there have been a lot of deep investigations in the mechanism of nasal mucosa damage-repair process after machinery injury. Several studies have supplied some knowledge, but the studies of nasal mucosa injury by light and heat have not been reported yet. VEGF, EGF, and FGF play important roles during nasal mucosa damage-repair process after machinery injury. We explored VEGF, EGF, and FGF expression and pathological changes, in order to get a deeper understand of the whole course of nasal mucosa injury by light and heat.The light and heat from nasal endoscope may be thought to be a destructive factor to nasal mucosa, which can lead to changes of damage-repair factors in damaged tissues. In this... |
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