The Protective Effect Of NMT Of TCM On Microvessel Basement Membrane Injury Of Cerebral Ischemia Reperfusion In Aged Rats

Ischemia Stroke is one of the dangerous,emergent,seriousdiseaseinclinic,its episode has notonlycomplexpathologicalbackground,but alsodangerous,multivariated,acuteprocedure,and itsmorbidity and mortality increase,with age growing old.In our country,with the increasing oldpopulation and the coming of senile society,it is important and urgent to study pathogenesis ofischemia cerebrovascular disease in Old-Age and explore effective therapeutics. Ischemical reperfusion injury is important pathophysiological mechanism causing manycerebrovascular diseases,resistingcerebralischemicalreperfusioninjuryis effective therapy measurein curing cerebral infarction.Two principal protease systems that can hydrolyze ECM are matrixmetalloproteinase system and plasminogen activator system in mechanism of ischemical reperfusioninjury.At present,from alteration of ECM components and protease,dyanmicly investigatingpathophysiological mechanism of cerebral ischemia reperfusion microvessel injury are few,especially,investigationinOld-Ageanimalsisnotstillreported.At present,young animals are usedin the major experimental study of sensile ischemic cerebral vascular diseases,then the importance ofaging factor in human cerebral micvascular diseases is ignored, which results in the larger differencebetween the study of experiment and clinic.Afew experimental findings about the recipes and drugswhich are used to treated the ischemic reperfusion injury of aged by detoxicating and removing turbidpathogenic factors,supplementing qi and promoting blood flow was reported, especially few studiesabout such recipes and drugs using to have the influence on the changes of elements in micvascularbasement membrane and on the expression correlating to degradative enzyme system caused byischemicreperfusioninjurywerereported.Astopresentcondition,thestudy is pointed to the injury ofmicrovascular extracellular matrix caused by ischemic reperfusion in the aged rats and is also pointedto the TCM theory of deficiency of qi and blood stasis, turbid poison to brain collateral.From thechange of certebromicrovessel structure and the content of type IV collagen and laminin,theexperiment suggests the characteristic of cerebral ischemia reperfusion injury in aged SD rats.Fromthe change of MMPs,TIMPandPA,PAIexpressionofcerebromicrovessel basement membrane,the experiment reveals the mechanism of cerebromicrovessel basement membrane injury of cerebralischemia reperfusion.Fromthechange ofstructureofcerebromicrovessel,and the content of type IVcollagen and laminin,it will confirm that NMT has the protective effect on the cerebral ischemiareperfusioninjuryinagedrats.Fromthechange ofMMPs,TIMPand PA,PAI ofcerebromicrovesselbasement membrane,it will reveal mechanism of protective effect on cerebral ischemia reperfusionmicrovessel injury.1 Character of cerebal ischemia reperfusion injury in aged rats and the protective effect ofNaomaitong ofTCM This experiment adopted animal model of focal cerebral ischemia 3h and reperfusion 6h,12h,24h,3d,6dinrats with MCAO method.Young SD rats andAged SD rats were divided randomlyintosham-operatedgroup inYoung SDrats,and model group inYoung SD rats(cerebral ischemia 3h英文摘要 7andreperfusion6h,12h,24h,3d,6d),sham-operated group inAged SD rats,model group inAged SDrats(cerebral ischemia 3h and reperfusion 6h,12h,24h,3d,6d),NMT middle dosage group(dividedinto groups ditto,0.90g·kg-1·d-1) in Aged SD rats,Nimodipine group(divided into groups ditto,6.00mg·kg-1·d-1)in Aged SD rats,NMT big dosage group(only observing reperfusion 24h,3d,1.80g·kg-1·d-1)in Aged SD rats,NMT little dosage group(only observing reperfusion24h,3d,0.45g·kg-1·d-1)inAged SD rats.Appling general observation (including neural function score,watercontent of cerebral constitution,area of cerebral infarction etc),commonly morphologic methods(includinghematoxylin-eosinstain,transmissionelect...