The Study On Interstitial Cells Of Cajal Changed In Mouse Colon Of Slow Transit Motility And The Role Of Nitric Oxide And Carbon Monoxide

Fuctional constipation(FC) is one of the commonest diseases in gastrointestinal tract. It plays an important role in colonic cancer, mammary cancer, hepatic encephalopathy, presenile dementia etc. FC is often fatal inducement of some acute cerebrovascular accident and cardiovascular disease.Recently, incidence of FC has been increased in the world, especially in the developed countries. FC has been divided into three types: slow-transit constipation(STC), outlet obstruction constipation (OOC), and mixed constipation. STC, about 45.5% of FC, is the obstipation characterized by lower colon motility. There are complex pathogenesis of STC, maybe related to more large and effective pushing forward contractions being reduced. These abnormal contractions maybeattribute to complex factors such as enteric nervous system(ENS), interstitial cells of Cajal(ICC), smooth muscle and neurotransmitters etc.The known functions of these ICC are their role in pacemaker generation, and serve as mediators interposing between enteric nerves and smooth muscle cells, which play an important complementary role in neurotransmission and control gastrointestinal motility. The abnormal number and ultrastructure of ICC lead to a variety of clinical disorders including gastrointestinal tumors and diseases of gastrointestinal motility.Some inhibitory neurotransmitters such as nitric oxide and carbon monoxide are produced by NANC system, and they always regulate gastrointestinal motility.However, the role of ICC and/or NO,CO in the mechanisms of STC are still largely unknown. To establish of STC animal model and investigate the role of ICC and correlated factors in order to understand their relationship in STC.PART-Ⅰ Establishment of mouse model with colon slow transit motility[OBJECTIVE] To establish a mouse model of colon slow transit motility, and investigate the pathophysiological mechanism of slow transit constipation.[METHODS] The mouse model was established by subcutaneous injection of morphine. According to dosage of morphine injected and periods of test, the mice were divided into severel groups, see tables:[RESULTS] 1. Fecal weight daily:(1) After 45 days: Compared with control groups, group I and group II was decreased (P<0.05), but there were no difference between group I and group II(P>0.05)(2) From 45 to 60 days:① Untreatment groups (group I a and group II a) were lower than control group a (P<0.01); there were no difference between group I a and group II a (P>0.05 ) .②there were no difference among the groups given naloxone( group I B , group Iγ , group II B)and control groups( P>0.05 ), while group II γ was lower than group I γ , control group γ (P<0.05 ),and no difference between group I (3 and group I γ (P>0.05 ), group II γ was lower than group II β (P<0.05); Compared with the untreatmentgroups (group I a , group II a ) , the groups given naloxone increased (P<0.01); (D there were no difference among control groups (P>0.05 ).2. The fecal character: in 45 days, all test groups(Bristol: 1-2) were more dry and hard than control groups(Bristol: 4-5); from 45 to 60 days, there was no improvement in all untreatment groups, while all groups using naloxone improved slightly.3. Intestinal transit rate:(1) On the 45 day, group I and group II were lower than control group (P<0.05 ) , while there was no difference between group I and group II (P>0.05)( 2 ) From 45 to 60 days: ①.there were no difference among control groups (control a, β and y) (P>0.05). ②. in the untreatment groups: group I a and group II a were lower than control group a (P<0.01); while there was no difference between group I a and group II a (P>0.05 ) .③there was no difference among groups given naloxone ( group I β, group I γ) and control group (P>0.05 ), and no difference between group I β and group I γ (P>0.05 ); while group II β was more decreased than control group P and group I β (P<0.05 ); and group II γ was lower than control group γ and group I γ (P<0.05). Comparedto untreatment groups (group I a , group II a ) , groups...