The Molecular Mechanism Of The Apoptosis In Hep-2 Cancer Cells Induced By Resveratrol

IntroductionResveratrol has been shown to have anticancer activities in both cell culture and animal carcinogenesis models. In this study we examined the effect of resveratrol on growth and induction of apoptosis of human laryngeal cancer cell line, Hep -2. MTT assay was used to determine the cell growth inhibitory rate. DNA fragmentation analysis and Hoechest staining method were used to quantitatively and qualitively detect the apoptosis status of Hep - 2 cells before and after the resveratrol treatment. Western blot was used to detect the expression of apoptosis - regulated gene caspase 3. We found that resveratrol strongly inhibited Hep - 2 cells proliferation in a time - and dose - dependent manner. In addition, induction of apoptosis was demonstrated by the appearance of chromatin condensation and DNA fragmentation. The activation of caspase - 3 and the specific proteolytic cleavage of poly ADP - ribose polymerase were detected during the course of apoptosis induction. These results suggest that resveratrol inhibited the human laryneal cancer cell proliferation by inducing cell apoptosis. As a chemotherapeutic agent resveratrol might have the effect against laryngeal carcinoma .Resveratrol is known to elicite vital cellular responses such as cell cycle arrest, apoptosis and differentiation by activating a cascade of molecular events. As there is an increasing interest to improve the efficacy of this compound for use as potential chemopreventive agents, we wanted to understand the impact of resveratrol on target genes in laryngeal cancer. In this study we used human cD-NA microarrays with 8000 human genes to characterize alterations in gene expression pattern in response to resveratrol. Over a 24 - hr exposure of Hep - 2...