Effect On Protective Inhibition Of Bone Marrow By "Sen Qi Gu Ben Fang" Toward Cancer After Chemical Treatment In Terms Of Clinical Signs And Experimence

Study purpose: Pass the clinical studying with experiment, study "Shen qi gu ben fang" for protection of the function of the tumor chemotherapy empress marrow represses.1.CIinical researchMethod: This study brings into 59 case lung cancer, through certain diagnosis by pathology or cytology, hospitalization which combine the lung cancer and large intestine cancer accepts the chemotherapy empress.lung cancer: non small cell lung cancer(NSCLC) a plan GC(gemcitabine+ciplastin), small cell lung cancer(SCLC) a plan CAV(cyclophosphamid + doxurubicin + vincristine);large intestine cancer: FOLFOX (oxaliplatin + calcium folinate + fluorouracil 5-FU) a plan, random component to 2 groups: Treatment group of 30_patients, control group of 29 patients. A plan of treatment: treatment group take orally "shen qi gu ben fang"200ml/time,bid x 28day/ course of treatment x 2;Control group take orally"Zhen qi fu zheng jiao nang" 3#/time, bid x 28day/ course of treatment x 2. two groups would take 3 weeks for prior treatment to observe the process of using drug for increasing leucocyle After chemotherapy two group to start using. Determination index: condition physical strength and objective sign of patient;count peripheral blood leucocyte and count neutral cell, hemoglobin, plastocyte;index of immunization: CD⁺3;CD⁺4;CD⁺8;CD⁺4/CD⁺8;fos-related antigen, CT/MRI, ECHO and function liver, function renal etc..Result: The clinical symptom of study group tumor chemotherapy empress myelosuppression and clinical symptom of control group all anesis obviously (p < 0.001) , the clinical symptom of study group to be in comparison with control group it should show that to anesis obviously, difference contain covariance meaning (p < 0.05) .The clinical symptom of study group (Karnofsky accumulated points) comparison with control group it should show that o effective power (p < 0.05). To change of the blood elephant index: The besides plastocyte (before and after normal) , leucocyte, neutral cell, hemoglobin and red blood cell of study group after treatment compare before treatment difference contain covariance meaning;( p < 0.01). The leucocyte cell, hemoglobin of the control group aftercuring before comparing the treatment go up difference contain covariance meaning ( p < 0.05;p < 0.01) ,the other index sign variety haven'nt covariance meaning( p>0.05). Study the set goes up obviously the lympho T subset cell a CD3+> CD/, and CD^/CDs difference contain covariance meaning (p < 0.05;p < 0.01;p < 0.01) , the CD/ and CD/VCDg* of study group compares with matched control group to go up obviously, difference contain covariance meaning ( p<0.001), fos-related antigen of two sets all descends and obviously( p< 0.01;p< 0.05) but two in comparision to haven't covariance meaning ( p > 0.05). Tumor stability rate(96%) that study the set is higher than matched control(83.1%), difference contain covariance meaning.( p < 0.05).Conclusion:"Shen qi gu ben fang" at the clinical obvious improvement clinical symptom of tumor chemotherapy empress the myelosuppression: Tired, julior word, white facial expression ,decreased food appetite, lumbar vertebrae ache, spontaneous perspiration and night sweat, dizziness and tinnitus. The physical strength condition Karnofsky is obvious increasing, increasing to exist the quantity, Go up the outside a blood: WBC, S N, Hb, RBC."Shen qi gu ben fang" at the clinical increase the immunity function of the human, special CD/ and CD/yCDs is clearer than matched control Show to go up difference contain covariance meaning( p < 0.05;p < 0.01)"Shen qi gu ben fang" at the clinical have the certain repressing tumor and antidotal degrees, may pass the exaltation the immunity function of the human, to transfer anti-cancer ability of organism.2. Experiment researchMethod: 60 a small rat ( small rat in the department of C57, bisexual and each half) Random component to 5 groups: "Shen qi gu ben fang " group, Cyclophosphamid + "Shen qi gu ben fang " group, Cyclophosphamid +"Zhen qi fu zheng jiao nang" group, Cyclophosphamid group, isotonic Nachloride matched control group, each 12.Spread 3 on behalfs sentence to death the small rat behind, The each small right side of the small rat armpit skin descends to inoculate a tumor suspension cell 0.2ml.Beginning Chinese herbal medicine to stomach in second day after inoculating, each group 0.5ml physic liquor in once a day, continuous 10 days. After inoculating, in addition to physiology brine matched control and a "Shen qi gu ben fang " group, all groups at give the medicine 4 days add the belly cavity injects. Cyclophosphamid (CTX) 80 mg/ kg/ d totally 3 days. Observe index sign: inhibition tumor rate, peripheral blood cells, lympho T subset cell, immune an organ weigh, cell factor TNF- a.Result:Outside a blood cell: "Shen qi gu ben fang " group, "Shen qi gu ben fang "+ Cyclophosphamid group, "Zhen qi fu zheng jiao nang" + Cyclophosphamid groupincomparision_with pure chemotherapy create differences between all contain covariance meaning.( p < 0.01;p < 0.05;p < 0.05), 2 with 3 group haven't covariance meaning a compared diffirence _{p > 0.05}. Inhibition tumor rate: "Shen qi gu ben fang "+ CTX group heavy ratio in lump comparisons "Zhen qi fu zheng jiao nang" + CTX group is obvious to alleviate( p < 0.05).Immunity organ weight: the spleen of "Shen qi gu ben fang " group are the most heavy in comparision with pure chemotherapy set, create difference between contain covariance learn the meaning( p < 0.01). "Shen qi gu ben fang "+ CTX group, "Zhen qi fu zheng jiao nang"+ CTX group in comparision with pure chemotherapy set create_diffences between all haven't contain covariance meaning( p > 0.05). bearing cancer pure using the "Shen qi gu ben fang " group a thymus gland is the most heavy, with the chemotherapy comparison difference contain covariance learn the meaning( p < 0.01).Serum TNF- a :The bearing cancer using "Shen qi gu ben fang "+ CTX group goes up the most obvious, with NS matched control compared diffence the covariance learn the meaning( p < 0.01), Compare with the pure chemotherapy set to also go up but haven't covariance learn the meaning( p > 0.05). "Shen qi gu ben fang "+ CTX group the level of TNF- a supports with "Zhen qi fu zheng jiao nang"+ CTX group goes up, difference contain covariance learn the meaning(p < 0.05).LymphoT subset cell: CD3+ level: "Shen qi gu ben fang " group, "Shen qi gu ben fang "+ CTX group with brine matched control in comparision with to all show to go up, differences between all contain covariance meaning( p < 0.01), with pure chemotherapy set also to go up obviously( p < 0.01). "Shen qi gu ben fang "+ CTX group with "Zhen qi fu zheng jiao nang"+ CTX groupin comparision with the defference hasn't the covariance meaning( p > 0.05). CD/ level: "Shen qi gu ben fang " group goes up at the most obvious, "Shen qi gu ben fang "+ CTX group, "Zhen qi fu zheng jiao nang"+ CTX group compares with pure chemotherapy that set all going up (p < 0.01) , with brine physiology matched control for the difference contain covariance meaning( p < 0.05).Conclusion:? " Shen qi gu ben fang " is the model which small rat Lewis lung cancer marrow repress goes up the leucocyte in a blood in outside, lymphoT subset cell and immunity organ weight( spleen, thymus gland weight).? " Shen qi gu ben fang " the model that small rat Lewis lung cancer marrow repress goes up the cell factor TNF- a, It is certain to repress the degree of the tumor, repressing rate is 30.05%. Special in restraint of tumor rate of "Shen qi gu ben fang "+ CTX with"Zhen qi fu zheng jiao nang"+ CTX to go up(90.02%;84.14%) obviously differentiate contain covariance meaning( p < 0.05).