| Benign Prostatic Hyperplasia (BPH) is a kind of frequent male old-age disease which clinical feature is urine difficulty. Along with the Cellular proliferation in prostate gland transitional zones, the physical volume of prostate gland enlarges continuously, thus oppresses the urethra part of prostate gland, leads to bladder outlet obstruction (BOO), the patients appear to be different degree urine difficulty symptom, the serious ones will suffer accumulated water in both sides of upper urinary tract and cause renal inadequacy. Therefore, as a frequent disease of male old people, BPH has already affected the life and the quality of life(QOL) of the old age male seriously. Along with progress of the people' s living standard and health care services, the aging population in our country increases gradually, the cases of BPH also increases correspondingly , BPH has already been a important topic in Chinese old-age medical science. The folks use ZiHuaMoLi to cure old age BPH, and get a certain curative effect in clinical application, Based in the medicine function of the ZiHuaMoLi, we launch this research to verify the medicine efficacy of ZiHuaMoLi, and explore the function mechanism of ZiHuaMoLi.1. Documents researchThe article reviews near 10-year modern Chinese medicine documents about this disease, and regards the foundation of this disease asdeficiency of kidney' Qi, the pathogenesis is blood—stasis and coagulated phlegm, blood stasis due to qi deficiency , lung qi loses dispersing also pathogenesis for this disease, so the therapeutical principle shall give consideration to deficiency and excess, treating both manifestation and root cause simultaneously. During the clinical treatment, curing it with differentiation of symptoms and signs for classification of syndrome, draw up particular prescription follow the variation of diagnosis, Chinese patent medicines and others therapy. In empirical study, we discuss the possible mechanism of curing BPH with traditional Chinese medicine mainly from affecting uropoiesis system smooth muscle tension , influencing the sex hormone' s level, contracting the prostate gland' s physical volume > repressing the prostatic cell' s reduplication and inducing prostatic cell' s Apoptosis. Practice certifies that a certain curative effect can be obtained in curing BPH with traditional Chinese medicine, but it also need to take into arrangement and exaltation at the same time, under the condition that the traditional mechanism of Chinese medicine can't be clarified completely, we ought to think more highly of rigorous clinical proof.Currently the modern medical science thinks that this disease' s outbreak reason involves the link such as sex hormone, growth factor and food, the medicine treatment is a developing tendency, clinical medicine curing BPH mainly includs 5 a -reducing enzyme inhibitor, a ,- adrenergic receptor antagonist, androgenic hormone inhibitor, galenical preparations ( include the plant Preparations and the pollen Preparations) etc. 5 a-reducing enzyme inhibitor is slow-moving since the effect, the side reaction is also obvious, the therapy expenses is higher too. As to a i~ adrenergic receptor antagonist, seeing from patients individual, not all patients can obtain the obvious curative effect. As far as the plant preparations and the pollen preparations are concerned, their mechanisms are still not quite clear. About the anti- androgenic hormone medicine, it will cause the level of testosterone in blood plasma to descend significantly, the curative effect is not good and side effect is not a few. Although some new medicine have entered the clinical application and the verification stages, but as to cure BPH, these medicine still exist many disadvantages such as inaccurate curative effect, big side effectand expensive cost.2, Experiment research(1) max administration experiment :In order to prove the clinical application safety of ZiHuaMoLi, we carries out the acute toxicity experiment, infuses The NIH small rat with 35 g(crude drug) / ml /once, the max administration of ZiHuaMoLi water-lifted liquid is the 30 ml/ kg ( the 1050.0 g crude drug / kg), namely the LD50 is more than 1050.0 g crude drug / kg. It is 628. 74 times more than the clinical day dosage( the 1.67 g crude drug / kg). Finally did not find the death of the animal or the abnormality of other internal organs. As a result , the ZiHuaMoLi water-lifted liquid is a kind of Chinese drugs preparation that takes orally safely.(2) observation of ZiHuaMoLi ' s function to anti-experimental BPH: In order to approach to the effect difference between ZiHuaMoLi and Proscar for BPH. We make the small rat BPH model with testosterone propionate hypodermical injection, by using this models we take intragastric administration difference with ZiHuaMoLi high dose , ZiHuaMoLi low dose, and Proscar , after the experiment, separate the small rat' s prostate gland tissue, measure the prostate gland wet weight, examine the small rats' testosterone (T) and serum androstanolone (DHT) content respectively, respectively observe the anti-BPH function and mechanism of ZiHuaMoLi high dose group, ZiHuaMoLi low dose group, and Proscar group .Result: Making the small rat BPH model with testosterone propionate hypodermical injection;Under the condition of owning the sex gland, the ZiHuaMoLi contain the anti- BPH function, at the same time there is no obvious difference of anti-BPH among ZiHuaMoLi high dose group, ZiHuaMoLi low dose group, and Proscar group. The mechanism of ZiHuaMoLi in inhibiting from BPH could be the same as Proscar, that' s inhibiting the transformation from testosterone to be androstanolone. But as for inhibiting the size of the prostate, the effect is less stable than Proscar, maintaining factors of unstable and variation, the anti-BPH function of ZiHuaMoLi is mostly repressing the transform of the testosterone to be androstenalone.(3) Effect of ZiHuaMoLi to prostate gland hyperplastic tissue' s Ki67, CD34 antigen presentation: Through the experiment of HE dyeing foreach prostate gland tissue, carry out the immunohistochemistry examination simultaneously, observe the prostate gland hyperplastic tissue' s Ki67> CD34 antigen presentation , the result shows there is not Ki67 antigen presentation in the normal small rats' prostate gland tissue , but the Ki67 antigen presentation rate in hyperplasy group and negative control group are obviously higher than Proscar group and ZiHuaMoLi high, low dose group , it shows the ZiHuaMoLi can lower Cellular proliferation and lower activity of cell multiplication, the anti-BPH mechanism of Proscar is similar to ZiHuaMoLi.At the same time the Ki67 and the MVD values present direct correlation, it hints that the expression of Ki67 and CD34 is direct correlation, the ZiHuaMoLi and the Proscar both have the function to lower the index of Ki-67 and CD34. MVD values in the the small rat' s prostate Interstitial tissue is higher than control group, hints that the BPH can be regarded as a kind of new-born vessels dependence disease. Supposing that MVD values increment in BPH small rats is relevant to vasiformation, is shows the Proscar can anti-BPH by repressing the generation of prostate gland tissue' s new-born vessels, thus keep prostate gland from hyperplasia, so attain the goal of prevent BPH. In general , the mechanism of the ZiHuaMoLi is similar to The Proscar.
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