Experimental Study On Replacement Of Abdominal Aorta With A Tissue Engineeded Patch

The concept of tissue engineering as it applies to heart valves or grafts is to transplant autologous cells onto a biodegradable scaffold in the shape of a heart valve or graft. After attachment, the cells form extracellular matrix material as the polymer simultaneously degrades, and create a viable, autologous valve or graft that can be transplanted into the same patient from whom the cells were harvested. The tissue-engineered valve or graft would have the potential to grow, remodel, and avoid infectious and thrombogenic complications. With autologous cells and biodegradable copolymer scaffold, some laboratory had been created autologous pulmonary valve leaflets and pulmonary artery, but seldom successful reports in aortic systemic. The present study makes some progress on seeding cell isolation and culture. Evaluates the biocompatibility of p(3HB-co-3HH)/PU, a new copolymer scaffold. Creates a tissue-engineered patch and evaluates whether the patch can withstand systemic pressure and can be used to create a vascular autograft for use in the aortic position. 1. Seeding cell isolation, culture and identifyObjective To find a simple and effective method for harvesting myofibroblasts and endothelial cells from one segment of femoral vena.