Experimental Studies On Gene Therapy Of Human Pancreatic Carcinoma

Human pancreatic carcinoma (HPC) is one of the malignant tumor which are very difficult in both diagnosis and treatment. Because of the lack of early detecting method, most of the HPC cases diagnosed are in advanced stage of the disease. Tumor resectability is therefore very low, and other non-operative approaches are also not encouraging. The prognosis of HPC is very poor and the mortality is very high. The aim of our researches was to investigate the gene therapy of HPC experimentally, and try to pave the theoretical and practical ground for the clinical gene therapy of HPC.Our studies included two parts: A). Constructing the recombinant retroviral vectors expressing antisense c-myc or antisense Ki-ras, and observing the effects of recombinant retroviral vectors on retardation of HPC cell growth in vitro and reversion of malignant phenotype of HPC. B). Constructing a recombinant retroviral vector expressing the "suicide" gene (HSV-TK) , and examining the killing effect of the HSV- TK gene on HPC cells ( tumor cells "suicide").1. Construction of the recombinant retroviral vectors(1).A 1.35Kb c-myc fragment containing the third exon and adjoining flanking intron sequences was inserted into the retroviral vector pXT1 in both sense and antisense orientation, the recombinant vectors were defined as pXT1Sm (sense) and pXTlAm (antisense).The recombinant vector DNA was introduced into PA317 amphotropic packaging cells. The HPC cell line (PC-2 )...